ZmBARK1 as a low-temperature tolerance gene in maize germination

文献类型： 外文期刊

第一作者： Xu, Qingyu

作者： Xu, Qingyu;Song, Yiying;Bao, Di;Di, Hong;Zhang, Lin;Dong, Ling;Zeng, Xing;Zhang, Jiayue;Li, Chunxiang;Xing, Jiapeng;Zhang, Naifu;Li, Xin;Wang, Zhenhua;Zhou, Yu;Meng, Lingzhi;Weng, Jianfeng

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关键词： Maize; Low-temperature; GWAS; RNA-seq; ZmBARK1

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2025 年 13 卷 4 期

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收录情况： SCI

摘要： Maize (Zea mays L.), a globally significant cereal crop, is produced in vast quantities worldwide. However, its growth is severely constrained by low temperatures, particularly during seed germination, which significantly impairs seedling emergence. In this study, genetic diversity across six germination-associated phenotypic traits (RGR, RSL, RTL, RRSA, RRV, and RSVI) of 304 inbred lines was analyzed, to evaluate the capacity of these lines for low-temperature tolerance. Genome-wide association study (GWAS) was carried out by combining six germination-associated phenotypic traits and genotypic data from 30-fold resequencing. The gene ZmBARK1 was identified through integrated GWAS and RNA-seq analyses, and its association with low-temperature tolerance during maize germination was validated by quantitative real-time PCR (qRT-PCR). ZmBARK1, encoding BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, was located on the bin 4.09 region of maize chromosome 4. Amino acid comparison and subcellular localization analyses revealed that ZmBARK1 is highly homologous to AtBAK1 and is localized to the plasma membrane of the cell, which may be involved in regulating brassinosteroid (BR) signaling. In addition, we revealed the role of ZmBARK1 in low-temperature tolerance during maize germination. Compared with wild-type (WT), the ethyl methanesulfonate (EMS) mutant zmbark1 was characterized by substantially enhanced low-temperature tolerance. Overall, these findings provide promising candidate genes, improve low-temperature tolerance in maize, and advance the understanding of regulatory mechanisms underlying maize's response to low-temperature stress. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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