An IgY Effectively Prevents Goslings from Virulent GAstV Infection

文献类型： 外文期刊

第一作者： Zhang, Mengran

作者： Zhang, Mengran;Gu, Youfang;Zhang, Mengran;Zhang, Lijiao;Yang, Jing;Zhao, Dongmin;Han, Kaikai;Huang, Xinmei;Liu, Qingtao;Xiao, Yichen;Li, Yin;Zhang, Mengran;Zhang, Lijiao;Yang, Jing;Zhao, Dongmin;Han, Kaikai;Huang, Xinmei;Liu, Qingtao;Xiao, Yichen;Li, Yin

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关键词： goose astrovirus; egg yolk antibody; prevention; treatment; passive immunoprevention

期刊名称：VACCINES （ 影响因子：4.961； 五年影响因子：5.325 ）

ISSN：

年卷期： 2022 年 10 卷 12 期

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收录情况： SCI

摘要： Goose astrovirus (GAstV) leads to viscera and joints urate deposition in 1- to 20-day-old goslings, with a mortality rate of up to 50%, posing a severe threat to entire colonies; however, there is no efficient prevention and control method for GAstV infection. This study describes a prophylactic anti-GAstV strategy based on the specific immunoglobulin Y (IgY) from egg yolk. The specific IgY was produced by 22-week-old laying hens intramuscularly immunized with the inactivated GAstV three consecutive times, with 2-week intervals. The egg yolk was collected weekly after the immunization and the anti-GAstV IgY titer was monitored using an agar gel immune diffusion assay (AGID). The results revealed that the AGID titer began to increase on day 7, reached a peak on day 49, and remained at a high level until day 77 after the first immunization. The specific IgY was prepared from the combinations of egg yolk from day 49 to day 77 through PEG-6000 precipitation. Animal experiments were conducted to evaluate the effects of prevention and treatment. The result of the minimum prophylactic dose of the IgY showed that the protection rate was 90.9% when 2.5 mg was administrated. Results of the prevention and the treatment experiments showed prevention and cure rates of over 80% when yolk antibody was administered in the early stages of the GAstV infection. These results suggested that the specific IgY obtained from immunized hens with the inactivated GAstV could be a novel strategy for preventing and treating GAstV infection.

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