Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Wang, Liuyang

作者： Wang, Liuyang;Yang, Xinling;Wang, Liuyang;Guo, Pei;Zhang, Xiaofang;Zhang, Tao;Wang, Liuyang;Ning, Jun;Duan, Yun

作者机构：

关键词： desaturases; unsaturated fatty acids; RNA interference; mating behavior; yeast expression; biosyntheticpathway

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 19 期

页码：

收录情况： SCI

摘要： In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6-85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Delta 11 and Delta 9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.

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