Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk

文献类型： 外文期刊

第一作者： Du, Bingyao

作者： Du, Bingyao;Du, Bingyao;Meng, Lu;Liu, Huimin;Zheng, Nan;Zhang, Yangdong;Zhao, Shengguo;Wang, Jiaqi;Du, Bingyao;Meng, Lu;Liu, Huimin;Zheng, Nan;Zhang, Yangdong;Zhao, Shengguo;Wang, Jiaqi

作者机构：

关键词： milk; PacBio single molecule real-time sequencing; psychrophilic bacteria; community structures; regional

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： In this study, we investigated the species composition and diversity of psychrotrophic bacteria in raw milk from Heilongjiang, Inner Mongolia, Gansu, Henan, Anhui, Jiangsu, Chongqing, and Hunan provinces in China using traditional cultivation and PacBio Single Molecule Real-Time sequencing methods. The isolated psychrotrophic bacteria were highly diverse, which composed of 21 genera and 59 species. Pseudomonas accounted for 58.9% of the total genera while Stenotrophomonas and Enterococcus were also highly represented (above 5.0%). In particular, P. azotoformans occurred at a level of 16.9% and P. paralactis, P. lactis, E. faecalis, and P. marginalis were present in relatively high proportions (above 4.0%). Regional differences were found significantly among the test regions except samples from Heilongjiang and Inner Mongolia were similar. Additionally, differences were observed between days in Henan, Anhui, and Jiangsu samples. Therefore, control strategies must be implemented on regional and season basis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序