Effects of Exogenous 24-Epibrassinolide Leaves Spraying Application on Chlorophyll Accumulation and Gene Expression Profiles of Chlorophyll Metabolism in Celery

文献类型： 外文期刊

第一作者： Chen, Chen

作者： Chen, Chen;Liu, Pei-Zhuo;Liu, Hui;Tao, Jian-Ping;Shu, Sheng;Xiong, Ai-Sheng;Wang, Li-Xiang;Shu, Sheng;Xiong, Ai-Sheng;Li, Meng-Yao;Tan, Guo-Fei;Zhang, Er-Jin;Zhou, Jian-Hua

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关键词： 24-epibrassinolide; chlorophyll accumulation; gene expression profiles; growth period; celery

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN：

年卷期： 2023 年 9 卷 12 期

页码：

收录情况： SCI

摘要： Celery is an important leaf vegetable crop in Apiaceae, of which the petiole and leaf blade are the main edible parts. The content and proportion of photosynthetic pigments, mainly chlorophyll, have an important effect on the growth and quality of celery. As a brassinosteroid (BR) plant hormone with high physiological activity, 24-epibrassinolide (24-EBL) has the physiological functions of promoting chlorophyll accumulation and delaying leaf senescence. To investigate the effects of 24-EBL treatment on chlorophyll accumulation at different growth stages of celery, celery plants (variety Ningqin NO. 1) were treated from 45 similar to 59 days after sowing (DAS), at intervals of 7 days, with two different concentrations of 24-EBL: 1.04 X 10(6) mol center dot L 1 and 1.67 x 10 6 mol center dot L-1. The content of chlorophyll and the expression levels of genes related to its metabolism were determined in celery leaf blades and petioles at three different stages (52, 59, 66 DAS). In the first stage (52 DAS), 1.04 x 10 (6) mol center dot L 1 treatment of 24-EBL increased the expression levels of genes related to chlorophyll biosynthesis (AgHEML, AgCHLG, and AgCAO) to promote the accumulation of chlorophyll in leaf blades. During the second and third stages (59 and 66 DAS, respectively), 1.67 X 10 6 mol center dot L (1) 24-EBL treatment induced the expression levels of genes related to chlorophyll cyclic regeneration (AgCLH) and inhibited the up-regulation of genes related to chlorophyll degradation (AgNYC, AgHCAR, and AgPPH) to promote chlorophyll (especially chlorophyll b) accumulation. These treatments regulated the ratio of chlorophyll a content to chlorophyll b content and changed the leaf color of the celery. The results show that leaf spraying with an appropriate concentration of 24-EBL can facilitate chlorophyll synthesis by promoting chlorophyll synthesis and cycling-related gene expression levels and increase chlorophyll content in the leaves of celery. This study provides a reference for exploring the specific function of 24-EBL in regulating chlorophyll content during the growth and development of celery.

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