The ERβ-cAMP signaling pathway regulates estradiol-induced ovine oocyte meiotic arrest

文献类型： 外文期刊

第一作者： Yang, Bingxue

作者： Yang, Bingxue;Zhao, Yufen;Yu, Kai;Weng, Yu;Du, Chenguang;Li, Haijun;Yang, Bingxue;Zhao, Yufen;Yu, Kai;Weng, Yu;Du, Chenguang;Li, Haijun;An, Yang;Yang, Yanyan;Yu, Boyang

作者机构：

关键词： Sheep; Cumulus-oocyte complex; 17 beta-estradiol; Cyclic adenosine monophosphate; Estrogen nuclear receptor

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： Although 17 beta-estradiol (E2) and its receptors (ERs) are reported to play important roles in regulating oocyte maturation, the specific mechanism remains unclear. First, we performed immunohistochemistry analyses to determine the expression of the ER alpha and ER beta proteins in ovine ovarian tissue. Second, E2 (0.5 ng/mL and 1 mu g/ mL) were added to pre-IVM medium for 0 h, 1 h and 2 h. The effects of E2 (0.5 ng/mL and 1 mu g/mL) on cyclic adenosine monophosphate (cAMP) level in cumulus-oocyte complexes (COCs) and on oocyte meiotic progression were evaluated by ELISA and DAPI staining respectively. Third, the effects of E2 on the gene and protein expression of ER alpha and ER beta in COCs were investigated by Western blotting and real-time PCR. Afterward, ER beta and cAMP regulators were added to the 2-h pretreatment medium with or without E2 (0.5 ng/mL) to explore the possible interactions among E2, cAMP and ER beta. The results showed that both ER alpha and ER beta proteins were expressed in ovine cumulus layers and oocytes. E2 significantly increased intra-COC cAMP levels, maintained oocyte meiotic arrest, and promoted ER beta transcript and protein expression. E2 treatment increased the cAMP concentration, which was enhanced by ER beta agonist treatment and remarkably attenuated by ER beta inhibitor treatment. Forskolin plus IBMX treatment increased ER beta protein expression in COCs (P < 0.05), and this was attenuated by Rp-cAMP treatment. In conclusion, E2 (0.5 ng/mL) increased intra-COC cAMP levels by promoting ER beta expression, thereby maintaining oocyte meiotic arrest. cAMP in COCs has a positive feedback effect on ER beta expression, which provides a novel explanation for the positive role of E2 in regulating ovine follicle development and oocyte maturation.

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