Coordinated regulation of Bacteroides thetaiotaomicron glutamate decarboxylase activity by multiple elements under different pH

文献类型： 外文期刊

第一作者： Liu, Shujun

作者： Liu, Shujun;Wen, Boting;Du, Guoming;Wang, Yulu;Ma, Xiaochen;Yu, Haiyan;Xin, Fengjiao;Zhang, Jingjian;Fan, Shilong;Zhou, Huan

作者机构：

关键词： Glutamate decarboxylase; Bacteroides thetaiotaomicron; pH -dependent; Regulation mechanism

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 403 卷

页码：

收录情况： SCI

摘要： Glutamate decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid, which plays a vital role in the gut-brain axis. Herein, a novel glutamate decarboxylase from Bacteroides thetaiotaomicron (BTGAD) was heterologously expressed. BTGAD possessed high catalytic efficiency at 60celcius and pH 3.6. As pH response, N -terminal sequence (NTS), C-terminal sequence (CTS), and beta-hairpin in BTGAD coordinately regulated its activity under different pH. NTS folded into a loop under acidic pH, and the truncation of NTS severely reduced its activity to 4.2%. While CTS occupied the active site under neutral pH and became disordered to release the inhibition effect under acidic conditions. The beta-hairpin, located near the active site, swung and formed open and closed conformations, which acted as an activity switch. This study provides the molecular basis for the coor-dinated regulation mechanism of BTGAD and lays a theoretical foundation for understanding the metabolism of dietary glutamate and its interaction relationships with the gut-brain axis.

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