UvPomt, an O-Methyltransferase Interacting with UvMAT1-1-3, for Regulating Growth, Stress Tolerance, and Virulence in Ustilaginoidea virens

文献类型： 外文期刊

第一作者： Li, Zhi

作者： Li, Zhi;Liu, Yongfeng;Li, Zhi;Yu, Junjie;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Wang, Shuchen;Qi, Zhongqiang;Du, Yan;Pan, Xiayan;Liu, Yongfeng

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关键词： rice false smut; Ustilaginoidea virens; fungal pathogenicity; fungi sexual reproduction; mating-type gene; O-methyltransferase

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2025 年 11 卷 6 期

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收录情况： SCI

摘要： Rice false smut (RFS), caused by Ustilaginoidea virens (teleomorph: Villosiclava virens), is a devastating fungal disease that severely impacts global rice production by reducing both yield and grain quality. While the mating-type gene UvMAT1-1-3 is known to regulate both sexual and asexual reproduction in U. virens, its regulatory mechanism remains unclear. In this study, an interacting protein of UvMAT1-1-3, a putative O-methyltransferase (UvPomt), was identified using yeast two-hybrid screening, and its interaction was further confirmed by co-localization microscopy. A quantitative reverse transcription PCR (qRT-PCR) analysis showed a significant up-regulation of UvPomt expression during the early infection stage of U. virens. Functional characterization revealed that Delta UvPomt mutants exhibited reduced fungal pathogenicity, vegetative growth, conidial production, and stress adaptation. Furthermore, a Western blot analysis revealed that the UvMAT1-1-3 protein level was reduced in Delta UvPomt mutants, whereas the UvPomt protein level was elevated in Delta UvMAT1-1-3 mutants. Taken together, these findings suggest a potential reciprocal regulation between UvPomt and UvMAT1-1-3. Understanding UvPomt's function could provide a potential molecular target for controlling RFS disease.

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