Rice N-biofertilization by inoculation with an engineered photosynthetic diazotroph

文献类型： 外文期刊

第一作者： Zeng, Yan

作者： Zeng, Yan;Wang, Mengmei;Cui, Lingwei;Li, Chang;Liu, Ying;Zheng, Yanning;Wang, Mengmei;Cui, Lingwei;Yu, Yunkai;Wang, Lida;Li, Chang

作者机构：

关键词： Fe-only nitrogenase; Photosynthetic diazotroph; Constitutive expression; Biofertilizer; Sustainable agriculture

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 0959-3993

年卷期： 2024 年 40 卷 5 期

页码：

收录情况： SCI

摘要： Photosynthetic diazotrophs expressing iron-only (Fe-only) nitrogenase can be developed into a promising biofertilizer, as it is independent on the molybdenum availability in the soil. However, the expression of Fe-only nitrogenase in diazotrophs is repressed by the fixed nitrogen of the soil, limiting the efficiency of nitrogen fixation in farmland with low ammonium concentrations that are inadequate for sustainable crop growth. Here, we succeeded in constitutively expressing the Fe-only nitrogenase even in the presence of ammonium by controlling the transcription of Fe-only nitrogenase gene cluster (anfHDGK) with the transcriptional activator of Mo nitrogenase (NifA*) in several different ways, indicating that the engineered NifA* strains can be used as promising chassis cells for efficient expression of different types of nitrogenases. When applied as a biofertilizer, the engineered Rhodopseudomonas palustris effectively stimulated rice growth, contributing to the reduced use of chemical fertilizer and the development of sustainable agriculture.

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