文献类型： 外文期刊

第一作者： Zhang, Jianchun

作者： Zhang, Jianchun;Kong, Jie;Cao, Jiawang;Dai, Ping;Chen, Baolong;Tan, Jian;Meng, Xianhong;Luo, Kun;Fu, Qiang;Luan, Sheng;Sui, Juan;Zhang, Jianchun;Kong, Jie;Cao, Jiawang;Dai, Ping;Chen, Baolong;Tan, Jian;Meng, Xianhong;Luo, Kun;Fu, Qiang;Luan, Sheng;Sui, Juan;Wei, Peiming

作者机构：

关键词： Penaeus vannamei; fecundity; mitochondria; EHF; PRDM9

期刊名称：BIOLOGY-BASEL （ 影响因子：4.2； 五年影响因子：4.4 ）

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年卷期： 2024 年 13 卷 4 期

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收录情况： SCI

摘要： Simple Summary: In order to investigate the molecular mechanisms responsible for the variations in reproductive capacity among Penaeus vannamei shrimp females, we analyzed individuals exhibiting significantly different spawning abilities. Subcellular variances in ovarian composition were observed in these distinct cohorts of females across different maturity stages. Additionally, we conducted a transcriptional-level screening of candidate genes and associated pathways implicated in ovarian maturation. Furthermore, we assessed the expression profiles of the divergent candidate genes EHF and PRDM9 at different stages of ovarian development, along with their cellular localization within the ovaries. These findings are anticipated to provide valuable insights into the molecular mechanisms underlying the differences in reproductive capacity among shrimp females. The Pacific whiteleg shrimp (Penaeus vannamei) is a highly significant species in shrimp aquaculture. In the production of shrimp larvae, noticeable variations in the reproductive capacity among female individuals have been observed. Some females experience slow gonadal development, resulting in the inability to spawn, while others undergo multiple maturations and contribute to the majority of larval supply. Despite numerous studies that have been conducted on the regulatory mechanisms of ovarian development in shrimp, the factors contributing to the differences in reproductive capacity among females remain unclear. To elucidate the underlying mechanisms, this study examined the differences in the ovarian characteristics between high and low reproductive bulks at different maturity stages, focusing on the cellular and molecular levels. Transmission electron microscopy analysis revealed that the abundance of the endoplasmic reticulum, ribosomes, mitochondria, and mitochondrial cristae in oocytes of high reproductive bulk was significantly higher than that of the low reproductive bulk in the early stages of ovarian maturation (stages I and II). As the ovaries progressed to late-stage maturation (stages III and IV), differences in the internal structures of oocytes between females with different reproductive capacities gradually diminished. Transcriptome analysis identified differentially expressed genes (DEGs) related to the mitochondria between two groups, suggesting that energy production processes might play a crucial role in the observed variations in ovary development. The expression levels of the ETS homology factor (EHF) and PRDI-BF1 and RIZ homology domain containing 9 (PRDM9), which were significantly different between the two groups, were compared using qRT-PCR in individuals at different stages of ovarian maturation. The results showed a significantly higher expression of the EHF gene in the ovaries of high reproductive bulk at the II and IV maturity stages compared to the low reproductive bulk, while almost no expression was detected in the eyestalk tissue of the high reproductive bulk. The PRDM9 gene was exclusively expressed in ovarian tissue, with significantly higher expression in the ovaries of the high reproductive bulk at the four maturity stages compared to the low reproductive bulk. Fluorescence in situ hybridization further compared the expression patterns of EHF and PRDM9 in the ovaries of individuals with different fertility levels, with both genes showing stronger positive signals in the high reproductive bulk at the four ovarian stages. These findings not only contribute to our understanding of the regulatory mechanisms involved in shrimp ovarian development, but also provide valuable insights for the cultivation of new varieties aimed at improving shrimp fecundity.

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