Transcriptomic and Metabolomic Investigation on Leaf Necrosis Induced by ZmWus2 Transient Overexpression in Nicotiana benthamiana

文献类型： 外文期刊

第一作者： Zhang, Xianwen

作者： Zhang, Xianwen;Luo, Biao;Wang, Fang;Wang, Yan;Li, Wanchang;Liang, Shuang;Zhou, Zhongjing;Bao, Jiandong;Song, Xijiao;Liao, Zhenfeng;Chen, Guang;Xu, Fei;Teng, Yi;Xu, Shengchun;Lin, Fu-Cheng;Luo, Biao;Bao, Jiandong;Lin, Fu-Cheng;Fang, Ruiqiu;Xu, Shengchun

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关键词： WUSCHEL; necrosis; transcriptomics; metabolomics; tobacco; plant transgenic technology

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 13 期

页码：

收录情况： SCI

摘要： WUSCHEL (WUS) is a crucial transcription factor in regulating plant stem cell development, and its expression can also improve genetic transformation. However, the ectopic expression of WUS always causes pleiotropic effects during genetic transformation, making it important to understand the regulatory mechanisms underlying these phenomena. In our study, we found that the transient expression of the maize WUS ortholog ZmWus2 caused severe leaf necrosis in Nicotiana benthamiana. We performed transcriptomic and non-target metabolomic analyses on tobacco leaves during healthy to wilted states after ZmWus2 transient overexpression. Transcriptomic analysis revealed that ZmWus2 transformation caused active metabolism of inositol trisphosphate and glycerol-3-phosphate, while also upregulating plant hormone signaling and downregulating photosystem and protein folding pathways. Metabolomic analysis mainly identified changes in the synthesis of phenylpropanoid compounds and various lipid classes, including steroid synthesis. In addition, transcription factors such as ethylene-responsive factors (ERFs), the basic helix-loop-helix (bHLH) factors, and MYBs were found to be regulated by ZmWus2. By integrating these findings, we developed a WUS regulatory model that includes plant hormone accumulation, stress responses, lipid remodeling, and leaf necrosis. Our study sheds light on the mechanisms underlying WUS ectopic expression causing leaf necrosis and may inform the development of future genetic transformation strategies.

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