miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles

文献类型： 外文期刊

第一作者： Yang, Xin

作者： Yang, Xin;Li, Zhenhui;Wang, Zhijun;Yu, Jiaao;Ma, Manting;Yang, Xin;Li, Zhenhui;Wang, Zhijun;Yu, Jiaao;Ma, Manting;Nie, Qinghua;Yang, Xin;Li, Zhenhui;Wang, Zhijun;Yu, Jiaao;Ma, Manting;Nie, Qinghua;Yang, Xin;Li, Zhenhui;Wang, Zhijun;Yu, Jiaao;Ma, Manting;Nie, Qinghua

作者机构：

关键词： microRNA; Cbl-b gene; mice; muscle atrophy

期刊名称：BIOMOLECULES （ 影响因子：6.064； 五年影响因子：6.191 ）

ISSN：

年卷期： 2022 年 12 卷 2 期

页码：

收录情况： SCI

摘要： As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene.

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