The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)

文献类型： 外文期刊

第一作者： Xu, Wannan

作者： Xu, Wannan;Li, Taiyue;Li, Jiahui;Liu, Siqi;Yu, Xing;Tang, Min;Dong, Jingxiu;Bu, Xingjiang;Xia, Xingquan;Nie, Liuwang;Xu, Wannan;Liu, Jianjun;Zhou, Huaxing

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关键词： Plestiodon elegans; karyotype; RNAseq; sex markers; sex chromosomes

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.3 ）

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年卷期： 2024 年 15 卷 6 期

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收录情况： SCI

摘要： The sex chromosomes of skinks are usually poorly differentiated and hardly distinguished by cytogenetic methods. Therefore, identifying sex chromosomes in species lacking easily recognizable heteromorphic sex chromosomes is necessary to fully understand sex chromosome diversity. In this paper, we employed cytogenetics, sex quantification of genes, and transcriptomic approaches to characterize the sex chromosomes in Plestiodon elegans. Cytogenetic examination of metaphases revealed a diploid number of 2n = 26, consisting of 12 macrochromosomes and 14 microchromosomes, with no significant heteromorphic chromosome pairs, speculating that the sex chromosomes may be homomorphic or poorly differentiated. The results of the sex quantification of genes showed that Calumenin (calu), COPI coat complex subunit gamma 2 (copg2), and Smoothened (smo) were at half the dose in males as in females, suggesting that they are on the X chromosome. Transcriptomic data analysis from the gonads yielded the excess expression male-specific genes (n = 16), in which five PCR molecular markers were developed. Restricting the observed heterozygosity to males suggests the presence of homomorphic sex chromosomes in P. elegans, XX/XY. This is the first breakthrough in the study of the sex chromosomes of Plestiodon.

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