Sensitive detection of genetically modified maize based on a CRISPR/Cas12a system

文献类型： 外文期刊

第一作者： Wang, Huimin

作者： Wang, Huimin;Su, Ailing;Xu, Shuping;Chang, Jingjing;Liu, Xiangguo;Liang, Chongyang;Xu, Shuping;Xu, Shuping

作者机构：

期刊名称：ANALYST （ 影响因子：4.2； 五年影响因子：4.1 ）

ISSN： 0003-2654

年卷期： 2024 年 149 卷 3 期

页码：

收录情况： SCI

摘要： With the vigorous development of biotechnology, genetically modified organisms (GMOs) have become more and more common. In order to effectively supervise and administrate them, the rapid and accurate detection of GMOs is urgently demanded. Here, GMO gene-specific sensing methods based on colorimetry and surface-enhanced Raman scattering (SERS) were proposed based on the lateral branch cleavage function of the CRISPR/Cas12a system. Two transgenes, pCaMV35S and M810 Cry1Ab, were chosen as targets for transgenic crops. By using these methods, we performed transgenic detection on five types of maize leaves and successfully distinguished transgenic from non-transgenic samples. The colorimetric method is rapid, economical and available for field detection. The SERS approach, giving a higher sensitivity to 100 fM, is more suitable for laboratory application scenarios. This study explores practical transgenic detection approaches and will be valuable for the supervision of GMOs. With the vigorous development of biotechnology, genetically modified organisms (GMOs) have become more and more common.

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