A Sec-dependent effector, CLIBASIA_04425, contributes to virulence in 'Candidatus Liberibater asiaticus'

文献类型： 外文期刊

第一作者： Zhang, Shushe

作者： Zhang, Shushe;Wang, Xuefeng;He, Jun;Fu, Shimin;Zhou, Changyong;Zhang, Shushe;Zhao, Tingchang;Zhang, Song

作者机构： Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing, Peoples R China;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China;Guangxi Citrus Breeding & Cultivat Engn Technol Ct, Guilin, Guangxi, Peoples R China

关键词： Citrus Huanglongbing (HLB); CLas; effector; CLas4425; cell death; virulence factor

期刊名称：FRONTIERS IN PLANT SCIENCE （ 2022影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide, mainly caused by 'Candidatus Liberibacter asiaticus' (CLas). It encodes a large number of Sec-dependent effectors that contribute to HLB progression. In this study, an elicitor triggering ROS burst and cell death in Nicotiana benthamiana, CLIBASIA_04425 (CLas4425), was identified. Of particular interest, its cell death-inducing activity is associated with its subcellular localization and the cytoplasmic receptor Botrytis-induced kinase 1 (BIK1). Compared with CLas infected psyllids, CLas4425 showed higher expression level in planta. The transient expression of CLas4425 in N. benthamiana and its overexpression in Citrus sinensis enhanced plant susceptibility to Pseudomonas syringae pv. tomato DC3000 & UDelta;hopQ1-1 and CLas, respectively. Furthermore, the salicylic acid (SA) level along with the expression of genes NPR1/EDS1/NDR1/PRs in SA signal transduction was repressed in CLas4425 transgenic citrus plants. Taken together, CLas4425 is a virulence factor that promotes CLas proliferation, likely by interfering with SA-mediated plant immunity. The results obtained facilitate our understanding of CLas pathogenesis.

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