文献类型： 外文期刊
第一作者： Niu, Canfang
作者： Niu, Canfang;Yang, Peilong;Yao, Bin
作者机构：
关键词： HAP phytase; Pepsin resistance; Trypsin resistance; Low PH; Optimizing the residual side chain; High activity
期刊名称：INOSITOL PHOSPHATES: METHODS AND PROTOCOLS
ISSN： 1064-3745
年卷期： 2020 年 2091 卷
页码：
收录情况： SCI
摘要： Phytases can catalyze the hydrolysis of indigestible phytate and releases the usable phosphorus. Protease resistance and high activity of enzymes facilitate their biotechnological and medical application. Here we described a genetic manipulation method to improve enzyme tolerance to pepsin, trypsin, and low pH by optimizing the residual side chain of trypsin- and pepsin-sensitive HAP phytase YeAPPA from Yersinia enterocolitica.
分类号：
- 相关文献
作者其他论文 更多>>
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者：Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词：high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者：Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词：
-
Cooperative Fermentation Using Multiple Microorganisms and Enzymes Potentially Enhances the Nutritional Value of Spent Mushroom Substrate
作者：Zhang, Anrong;He, Weizhao;Han, Yunsheng;Zheng, Aijuan;Chen, Zhimin;Meng, Kun;Yang, Peilong;Liu, Guohua
关键词：fermentation; Pleurotus eryngii; nutritional value; condition optimization; enzymatic treatment
-
Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
作者：Zhang, Ruohan;Zhang, Honglian;Tu, Tao;Luo, Huiying;Yao, Bin;Tian, Jian;Huang, Huoqing;Guan, Feifei;Tian, Xiaoqian;Zhang, Yan;Wu, Ningfeng;Tian, Jian;Tian, Xiaoqian;Zhang, Yan;Sun, Jilu
关键词：Chitin; Chitinase; Endo-activity; Mutants; Functional oligosaccharides
-
Exploiting Systematic Engineering of the Expression Cassette as a Powerful Tool to Enhance Heterologous Gene Expression in Trichoderma reesei
作者：Ji, Wangli;Xu, Xinxin;Zhang, Wei;Xu, Li;Sun, Xianhua;Zhang, Honglian;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Huang, Huoqing
关键词：heterologous gene expression; signal peptide; Kozak sequence; 3 '-UTR; Trichoderma reesei; mannanase
-
Lactiplantibacillus plantarum FRT4 attenuates high-energy low-protein diet-induced fatty liver hemorrhage syndrome in laying hens through regulating gut-liver axis
作者：Li, Daojie;Cai, Hongying;Liu, Guohua;Han, Yunsheng;Qiu, Kai;Liu, Weiwei;Meng, Kun;Yang, Peilong;Cai, Hongying
关键词：Fatty liver hemorrhage syndrome; Gut microbiota; Lactiplantibacillus plantarum FRT4; Laying hens; Lipid metabolism
-
Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions
作者：Wang, Lei;Yao, Bin;Yao, Junhu;Wang, Lei;Luo, Huiying;Yao, Bin;Zhang, Jie
关键词：alanine; Cupriavidus necator; cell density; hexose utilization pathway; metabolic engineering; poly-beta-hydroxybutyrate
