文献类型： 外文期刊
第一作者： Niu, Canfang
作者： Niu, Canfang;Yang, Peilong;Yao, Bin
作者机构：
关键词： HAP phytase; Pepsin resistance; Trypsin resistance; Low PH; Optimizing the residual side chain; High activity
期刊名称：INOSITOL PHOSPHATES: METHODS AND PROTOCOLS
ISSN： 1064-3745
年卷期： 2020 年 2091 卷
页码：
收录情况： SCI
摘要： Phytases can catalyze the hydrolysis of indigestible phytate and releases the usable phosphorus. Protease resistance and high activity of enzymes facilitate their biotechnological and medical application. Here we described a genetic manipulation method to improve enzyme tolerance to pepsin, trypsin, and low pH by optimizing the residual side chain of trypsin- and pepsin-sensitive HAP phytase YeAPPA from Yersinia enterocolitica.
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