Screening of host genes regulated by ID1 and ID3 proteins during foot-and-mouth disease virus infection

文献类型： 外文期刊

第一作者： Luo, Yingying

作者： Luo, Yingying;Wang, Guangxiang;Ren, Tingting;Chen, Haotai;Yin, Xiangping;Sun, Yuefeng;Li, Yanmin;Zhang, Zhidong;Zhang, Tianliang

作者机构：

关键词： ID1; ID3; FMDV; Host genes; Genes knockout

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2021 年 306 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease virus (FMDV) is an important pathogen that harms cloven-hoofed animals and has caused serious losses to livestock production since its discovery. Furthermore, inhibitor of DNA binding (ID) proteins have been thoroughly studied in tumorigenesis, differentiation and metastasis, but its role in viral infection is rarely known. In this study, three gene knockout cell lines ID1 KO, ID3 KO, ID1/3 KO were obtained based on BHK-21 cells. We found that ID1 and ID3 genes single or double knockout promote the replication of FMDV. Moreover, compared with negative control cells during virus infection, there were 551 up-regulated genes and 1222 down-regulated genes in the ID1 KO cell line; 916 up-regulated genes and 1845 downregulated genes in the ID3 KO cell line; 810 up-regulated genes and 1566 down-regulated genes in ID1/3 KO cell line. Further genes expression patterns verification results also showed a good correlation between the data of RT-qRCR and RNA-seq. These findings provide a basis for studying the relevant mechanisms between host genes and ID genes during FMDV infection.

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