Unraveling the Anthocyanin Regulatory Mechanisms of White Mutation in Verbena stricta by Integrative Transcriptome and Metabolome Analysis

文献类型： 外文期刊

第一作者： Chai, Shengyue

作者： Chai, Shengyue;Yang, Jiaming;Zhang, Xiaofei;Shang, Xuwen;Lang, Lixin

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关键词： Verbena stricta; mutation; transcriptome; pigmentation; anthocyanins

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

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年卷期： 2024 年 15 卷 12 期

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收录情况： SCI

摘要： Background: Verbena stricta is a perennial herb of the Verbenaceae family, known for its medicinal properties, wide adaptability, and high resistance. Methods: This research investigated the metabolic pathways of flower color change by combining transcriptome and metabolomics analyses. Results: In purple flowers and white variants, a total of 118 differentially accumulated metabolites (DAMs), including 20 anthocyanins, and 7627 differentially expressed genes (DEGs) were found. The downregulation of delphinidin-3-O-galactoside, delphinidin-3-O-glucoside, and delphinidin-3-O-(6 ''-O-p-coumaroyl) glucoside, along with the absence of petunidin and malvidin derivatives, may explain the loss of pigmentation in the white-flower mutant. Fourteen candidate genes involved in anthocyanin biosynthesis were identified, among which the expression of Flavonoid 3 ', 5 '-hydroxylase (F3 ' 5 ' H) was significantly downregulated, notably limiting flux through the delphinidin pathway and reducing delphinidin accumulation. This limitation in upstream reactions, coupled with the multi-shunt process in downstream reactions, completely blocked the production of petunidin and malvidin. Conclusions: These findings offer new opinions on the anthocyanin metabolites and key genes responsible for the floral pigmentation in V. stricta. Additionally, the white variant provides a valuable platform for future research into the ornamental flower color of the Verbenaceae family.

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