Binding properties of odorant-binding protein 4 from bean bug Riptortus pedestris to soybean volatiles

文献类型： 外文期刊

第一作者： Zhu, Xiu-Yun

作者： Zhu, Xiu-Yun;Zhang, Hui;Zhang, Hui-Ru;Zhang, Dong;Zhang, Xiao-Ya;Wan, Zhi-Wei;Li, Xiao-Ming;Zhang, Ya-Nan;Li, Jin-Bu;Li, Jin-Bu;Yin, Mao-Zhu;Liu, Jia;Dewer, Youssef

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关键词： bean bug; competitive binding assay; odorant-binding protein; site-directed mutagenesis; soybean volatiles

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.424； 五年影响因子：3.268 ）

ISSN： 0962-1075

年卷期： 2022 年 31 卷 6 期

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收录情况： SCI

摘要： The bean bug Riptortus pedestris is a notorious insect pest that can damage various crops, especially soybean, in East Asia. In insects, the olfactory system plays a crucial role in host finding and feeding behaviour in which the odorant-binding proteins (OBPs) are believed to be involved in initial step in this system. In this study, we produced the R. pedestris adult antennae-expressed RpedOBP4 protein using a recombinant expression system in E. coli. Fluorescence competitive binding confirmed that RpedOBP4 has binding affinities to 7 of 20 soybean volatiles (ligands), and that a neutral condition is the best environment for it. The binding property of RpedOBP4 to these ligands was further revealed by integrating data from molecular docking, site-directed mutagenesis and ligand binding assays. This demonstrated that five amino acid residues (I30, L33, Y47, I57 and Y121) are involved in the binding process of RpedOBP4 to corresponding ligands. These findings will not only help us to more thoroughly explore the olfactory mechanism of R. pedestris during feeding on soybean, but also lead to the identification of key candidate targets for developing environmental and efficient behaviour inhibitors to prevent population expansion of R. pedestris in the future.

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