Complement component 3 haplotypes influence serum complement activity and milk production traits in Chinese Holstein cattle

文献类型： 外文期刊

第一作者： Wang, Yonghui

作者： Wang, Yonghui;Zhai, Junyu;Sun, Yan;Li, Yuhua;Wang, Changfa;Yang, Chunhong;Wang, Jingpeng;Ju, Zhihua;Huang, Jingming

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.752； 五年影响因子：4.069 ）

ISSN： 1932-6203

年卷期： 2022 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Complement component 3 (C3) is the key molecule of the three pathways of complement activation (alternative, classical, and lectin pathways), which are involved in phagocytosis, inflammation, and immunoregulation processes to destroy infectious microorganisms. In this study, three novel single-nucleotide polymorphisms (SNPs) (g.-1293C>G located in the 5'-flanking region, g.56T>C in exon I, and g.7017C>T in exon XII) of the C3 gene were detected using created restriction site polymerase chain reaction, restriction fragment length polymorphism, and DNA sequencing in 952 cattle from three Chinese breeds. The genotypes and haplotypes were analyzed to investigate the polymorphisms and their possible implications, with particular investigative focus on their associations with serum C3 level, complement hemolytic activity (CH50 and ACH50), and milk production traits. The g.56T>C SNP in exon I affected the serum ACH50 (P<0.01) and the milk somatic cell score (SCS) (P<0.05), and the g.7017C>T SNP in exon XII significantly affected the serum ACH50 values (P<0.01). Moreover, statistical analyses revealed that individuals with genotypic combination CCC/GCC showed significantly lower SCS and the lowest C3 concentration in serum compared with cows with CCC/GTT (P= 0.0007) and CTT/CTT (P= 0.0021); the individuals with CCC/CCT had significantly higher ACH50 values than those with CCC/CTC (P = 0.0008) and CTC/GTC (P = 0.001); cows with CCT/CTT had higher values of CH50 and 305-day milk yield (P>0.05). The C3 expression levels were significantly increased in lung and mammary tissues (P<0.05), while significantly decreased in heart, spleen, liver, and kidney tissues in mastitis cows compared with those in healthy animals (P<0.01), respectively. Bacterial counts of serum antibacterial activities were also completed to verify the effect of SNPs on resistance to mastitis pathogens. Genetically resistant cows (CCC/GCC) had serum with noticeably higher antibacterial activity against S. aureus and E. coli in vitro than the genetically susceptible CCC/GTT cows (P<0.05). Results from this study imply that the C3 gene plays a role in resistance to bacterial infection and that it can be used as a molecular marker for complement activity and traits related to milk production.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Targeting Nrf2/KEAP1 signaling pathway using bioactive compounds to combat mastitis

    作者：Khan, Muhammad Zahoor;Li, Liangliang;Zhan, Yandong;Huang, Binjiang;Liu, Xiaotong;Kou, Xiyan;Wang, Tongtong;Wang, Changfa;Khan, Adnan;Qadeer, Abdul;Ullah, Qudrat;Alzahrani, Khalid J.;Zahoor, Muhammad

    关键词：mastitis; inflammation; immunity; oxidative stress; antioxidants; bioactive compounds; Nrf2/Keap1 signaling pathway

  • Effects of Different Light Spectra on Oxidative Stress and Nutritional Quality of the Fish Plectropomus leopardus

    作者：Li, Wensheng;Zhang, Zheng;Liu, Baoliang;Fang, Yingying;Cao, Shuquan;Li, Wenyang;Fei, Fan;Li, Wensheng;Liu, Baoliang;Sun, Yan;He, Chengbin;Zhang, Chuanxin

    关键词：light spectra; blood biochemistry; antioxidant stress; nutritional quality; Plectropomus leopardus; light spectra; blood biochemistry; antioxidant stress; nutritional quality; Plectropomus leopardus

  • Genome-Wide Identification and Expression Analysis Under Abiotic Stress of the Lipoxygenase Gene Family in Maize (Zea mays)

    作者：Li, Sinan;Hou, Shuai;Sun, Yuanqing;Sun, Minghao;Sun, Yan;Li, Xin;Li, Yunlong;Wang, Luyao;Cai, Quan;Guo, Baitao;Zhang, Jianguo

    关键词：maize; LOX; abiotic stress; gene family; expression analysis

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Biogeochemical niches modulate shoot and root biomass in Potentillinae across Chinese grasslands

    作者：Wang, Chao;Li, Xiaona;Hou, Yanhui;Suonan, Ji;Wang, Yonghui;Wang, Yonghui;Yu, Lingfei;Penuelas, Josep;Sardans, Jordi;Penuelas, Josep;Sardans, Jordi

    关键词：above- and below-ground; continental scale; ecosystem functions; elemental concentrations; Elementome; grassland; Hypervolume

  • Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation

    作者：Sun, Yan;Yan, Fang;Sun, Yan;Wu, Jiajing;Shen, Beilei;Luo, Rongbo;Zhang, Shijun;Li, He;Qian, Bingshuo;Fan, Lingjun;Zhang, Junkui;Wang, Tiecheng;Xia, Xianzhu;Gao, Yuwei;Yang, Hengzheng;Cui, Huizi;Han, Weiwei;Xia, Xianzhu

    关键词：TRPV4; anti-influenza; machine learning; repurposing drugs; molecular docking

  • EIDD-2801 resists to infection and co-infection of SARS-CoV-2 and influenza virus

    作者：Qian, Bingshuo;Luo, Rongbo;Shen, Beilei;Fan, Lingjun;Zhang, Junkui;Zhang, Shijun;Sun, Yan;Deng, Xiuwen;Gao, Yuwei;Qian, Bingshuo;Zhang, Junkui;Pang, Xiaobin;Zhong, Wu

    关键词：Antiviral activity; Respiratory viruses; Influenza; SARS-CoV-2; EIDD-2801; Co-infection.

微信小程序