Infection phase-dependent dynamics of the viral and host N6-methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo

文献类型： 外文期刊

第一作者： Zhuang, Guoqing

作者： Zhuang, Guoqing;Zhao, Xuyang;Jin, Jiaxin;Zhu, Xiaojing;Wang, Rui;Zhai, Yunyun;Lu, Wenlong;Sun, Aijun;Zhang, Gaiping;Zhuang, Guoqing;Zhao, Xuyang;Jin, Jiaxin;Zhu, Xiaojing;Wang, Rui;Zhai, Yunyun;Lu, Wenlong;Sun, Aijun;Zhang, Gaiping;Liao, Yifei;Teng, Man;Luo, Jun;Zhang, Gaiping;Teng, Man;Luo, Jun;Zhang, Gaiping;Teng, Man;Yao, Yongxiu;Nair, Venugopal;Yao, Yongxiu;Nair, Venugopal;Yao, Wen;Zhang, Gaiping

作者机构：

关键词： epitranscriptome; N6-methyladenosine (m(6)A); oncogenic viruses; tumorigenesis

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：12.7； 五年影响因子：8.5 ）

ISSN： 0146-6615

年卷期： 2023 年 95 卷 1 期

页码：

收录情况： SCI

摘要： Dynamic alteration of the epitranscriptome exerts regulatory effects on the lifecycle of oncogenic viruses in vitro. However, little is known about these effects in vivo because of the general lack of suitable animal infection models of these viruses. Using a model of rapid-onset Marek's disease lymphoma in chickens, we investigated changes in viral and host messenger RNA (mRNA) N6-methyladenosine (m(6)A) modification during Marek's disease virus (MDV) infection in vivo. We found that the expression of major epitranscriptomic proteins varies among viral infection phases, reprogramming both the viral and the host epitranscriptomes. Specifically, the methyltransferase-like 3 (METTL3)/14 complex was suppressed during the lytic and reactivation phases of the MDV lifecycle, whereas its expression was increased during the latent phase and in MDV-induced tumors. METTL3/14 overexpression inhibits, whereas METTL3/14 knockdown enhances, MDV gene expression and replication. These findings reveal the dynamic features of the mRNA m(6)A modification program during viral replication in vivo, especially in relation to key pathways involved in tumorigenesis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序