Development of a label-free photoelectrochemical immunosensor for novel astrovirus detection

文献类型： 外文期刊

第一作者： Shen, Quan

作者： Shen, Quan;Zhang, Wen;Shen, Quan;Qian, Lingling;Bao, Yingying;Wang, Xiaochun;Liu, Yuwei;Yang, Shixing;Ji, Likai;Zhang, Wen;Chen, Yun;Li, Henan;Wang, Jiangqiang;Shan, Tongling

作者机构：

关键词： AstV; Goose astrovirus; Prokaryotic expression; Polyclonal antibody

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：5.0 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 7 期

页码：

收录情况： SCI

摘要： Since 2017, an infectious goose gout disease characterized by urate precipitation in viscera, mainly caused by novel goose astrovirus (GoAstV) infection, has emerged in the main goose-producing region of China. The current challenge in managing goose gout disease is largely due to the absence of a rapid and efficient detection method for the GoAstV pathogen. Notably, the potential application of immunosensors in detecting GoAstV has not yet been explored. Herein, a label-free PEC immunosensor was fabricated by using purchased TiO2 as the photoactive material and antibody against GoAstV P2 proteins as the specific recognition element. First, we successfully expressed the capsid spike domain P2 protein of ORF2 from GoAstV CHSH01 by using the pET prokaryotic expression system. Meanwhile, the polyclonal antibody against GoAstV capsid P2 protein was produced by purified protein. To our knowledge, this is the first establishment and preliminary application of the label-free photoelectrochemical immunosensor method in the detection of AstV. The PEC immunosensor had a linear range of 1.83 fg mL-1 to 3.02 ng mL-1, and the limit of detection (LOD) was as low as 0.61 fg mL-1. This immunosensor exhibited high sensitivity, great specificity, and good stability in detecting GoAstV P2 proteins. To evaluate the practical application of the immunosensor in real-world sample detection, allantoic fluid from goose embryos was collected as test samples. The results indicated that of the eight positive samples, one false negative result was detected, while both negative samples were accurately detected, suggesting that the constructed PEC immunosensor had good applicability and practical application value, providing a platform for the qualitative detection of GoAstV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序