Lnc-MEG8 regulates yak myoblast differentiation via the miR-22-3p/RTL1 axis

文献类型： 外文期刊

第一作者： Ma, Xiaoming

作者： Ma, Xiaoming;La, Yongfu;Wang, Tong;Huang, Chun;Feng, Fen;Guo, Xian;Bao, Pengjia;Wu, Xiaoyun;Chu, Min;Liang, Chunnian;Yan, Ping;Ma, Xiaoming;Yan, Ping;Ma, Xiaoming;La, Yongfu;Wang, Tong;Huang, Chun;Feng, Fen;Guo, Xian;Bao, Pengjia;Wu, Xiaoyun;Chu, Min;Liang, Chunnian;Yan, Ping;Ma, Xiaoming;La, Yongfu;Wang, Tong;Huang, Chun;Feng, Fen;Guo, Xian;Bao, Pengjia;Wu, Xiaoyun;Chu, Min;Liang, Chunnian;Yan, Ping

作者机构：

关键词： Yak; Skeletal myoblasts; Lnc-MEG8; RTL1; MiR-22-3p

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe yak (Bos grunniens) is essential to the livelihoods of Tibetan people on the Qinghai-Tibet Plateau; however, its growth and productivity are constrained by the region's harsh climate and high altitude. Yak skeletal muscle myoblasts, which have evolved to thrive under these challenging conditions, offer a valuable model for investigating muscle development. In this study, we performed transcriptome profiling of yak longissimus dorsi muscle at different growth stages, identifying a key long non-coding RNA, LncRNA-XR_314844 (Lnc-MEG8), with a potential role in muscle development.ResultsWe developed a novel technique to isolate high-quality yak myoblasts, enabling detailed analysis of Lnc-MEG8. Our results indicated that Lnc-MEG8's subcellular localization varies during muscle cell growth: it is found in both the nucleus and cytoplasm during proliferation but shifts mainly to the cytoplasm during differentiation. Functional experiments showed that Lnc-MEG8 promotes cell proliferation and inhibits differentiation, while its silencing had the opposite effect. Further analysis revealed that both Lnc-MEG8 and the gene RTL1 share miR-22-3p as a common target. Dual-luciferase assays confirmed miR-22-3p directly targets both Lnc-MEG8 and RTL1 mRNA. Co-transfection of Lnc-MEG8 and a miR-22-3p mimic restored RTL1 expression, highlighting Lnc-MEG8's regulatory role. Lnc-MEG8 also counteracts miR-22-3p's suppression of key muscle genes such as MyF5 and MyoG, facilitating myotube formation.ConclusionThese findings demonstrate that the Lnc-MEG8-miR-22-3p-RTL1 axis plays a crucial role in yak muscle development, providing insights that could advance muscle tissue engineering and enhance yak meat quality.

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