Characterization of Terpenoids from the Ambrosia Beetle Symbiont and Laurel Wilt Pathogen Harringtonia lauricola

文献类型： 外文期刊

第一作者： Zhu, Zhiqiang

作者： Zhu, Zhiqiang;Yang, Chenjie;Liu, Sen;Pu, Huili;Chen, Yuxi;Qiu, Junzhi;Keyhani, Nemat O.;Jia, Peisong;Wu, Dongmei;Stevenson, Philip C.;Fernandez-Grandon, G. Mandela;Pan, Jieming;Guan, Xiayu

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关键词： Ophiostomales; Harringtonia lauricola; laurel wilt; secondary metabolite; terpenoid; antimicrobial; antiproliferative; free radical scavenging

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 12 期

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收录情况： SCI

摘要： Little is known concerning terpenoids produced by members of the fungal order Ophiostomales, with the member Harringtonia lauricola having the unique lifestyle of being a beetle symbiont but potentially devastating tree pathogen. Nine known terpenoids, including six labdane diterpenoids (1-6) and three hopane triterpenes (7-9), were isolated from H. lauricola ethyl acetate (EtOAc) extracts for the first time. All compounds were tested for various in vitro bioactivities. Six compounds, 2, 4, 5, 6, 7, and 9, are described functionally. Compounds 2, 4, 5, and 9 expressed potent antiproliferative activity against the MCF-7, HepG2 and A549 cancer cell lines, with half-maximal inhibitory concentrations (IC50s) similar to 12.54-26.06 mu M. Antimicrobial activity bioassays revealed that compounds 4, 5, and 9 exhibited substantial effects against Gram-negative bacteria (Escherichia coli and Ralstonia solanacearum) with minimum inhibitory concentration (MIC) values between 3.13 and 12.50 mu g/mL. Little activity was seen towards Gram-positive bacteria for any of the compounds, whereas compounds 2, 4, 7, and 9 expressed antifungal activities (Fusarium oxysporum) with MIC values ranging from 6.25 to 25.00 mu g/mL. Compounds 4, 5, and 9 also displayed free radical scavenging abilities towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide (O2-), with IC50 values of compounds 2, 4, and 6 similar to 3.45-14.04 mu g/mL and 22.87-53.31 mu g/mL towards DPPH and O2-, respectively. These data provide an insight into the biopharmaceutical potential of terpenoids from this group of fungal insect symbionts and plant pathogens.

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