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香蕉14-3-3蛋白基因Ma-14-3-3d的克隆及序列分析(英文)

文献类型： 中文期刊

作者： 李美英 1 ; 徐碧玉 1 ; 杨小亮 1 ; 刘菊华 1 ; 张建斌 1 ; 金志强 1 ;

作者机构： 1.中国热带农业科学院热带生物技术研究所;海南大学农学院

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2008 年 03 期

页码： 81-85

摘要： [Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.

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