文献类型： 中文期刊

作者： 李长青 ¹ ; 金海 ¹ ; 郭雪峰 ¹ ; 李华伟 ¹ ; 栗原光规 ¹ ;

作者机构： 1.内蒙古农牧业科学院生物中心;内蒙古农业大学动物科学与医学学院;日本国独立行政法人农业·食品产业技术研究机构畜产草地研究所

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2008 年 03 期

页码： 49-52+55

摘要： [Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. [Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. [Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. [Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat.