文献类型: 中文期刊
作者: 郇志博 1 ; 王金信 2 ;
作者机构: 1.中国热带农业科学院分析测试中心
2.山东农业大学植物保护学院
关键词: 无芒稗;质体ACCase;基因表达;Real-time PCR
期刊名称: 热带作物学报
ISSN: 1000-2561
年卷期: 2013 年 34 卷 09 期
页码: 1708-1713
收录情况: CSCD
摘要: 为研究一种抗精喹禾灵无芒稗的抗药性机理,建立了一种以β-actin为内参基因利用实时荧光定量PCR技术测定抗性无芒稗质体ACCase基因相对表达水平的方法。分析结果表明,设计的实时定量引物特异性非常好,内参基因、目的基因的扩增效率分别为103.2%、104.3%,线性相关系数分别为0.993 0、0.991 2,完全满足Real-time PCR定量方法的要求。所建立的无芒稗质体型ACCase基因Real-time PCR定量方法将为研究质体型ACCase基因的表达和杂草对ACCase抑制剂的抗药性机制研究提供方法学参考。
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