您好,欢迎访问中国热带农业科学院 机构知识库!

无芒稗质体ACCase基因Real-time PCR定量方法的建立

文献类型: 中文期刊

作者: 郇志博 1 ; 王金信 2 ;

作者机构: 1.中国热带农业科学院分析测试中心

2.山东农业大学植物保护学院

关键词: 无芒稗;质体ACCase;基因表达;Real-time PCR

期刊名称: 热带作物学报

ISSN: 1000-2561

年卷期: 2013 年 34 卷 09 期

页码: 1708-1713

收录情况: CSCD

摘要: 为研究一种抗精喹禾灵无芒稗的抗药性机理,建立了一种以β-actin为内参基因利用实时荧光定量PCR技术测定抗性无芒稗质体ACCase基因相对表达水平的方法。分析结果表明,设计的实时定量引物特异性非常好,内参基因、目的基因的扩增效率分别为103.2%、104.3%,线性相关系数分别为0.993 0、0.991 2,完全满足Real-time PCR定量方法的要求。所建立的无芒稗质体型ACCase基因Real-time PCR定量方法将为研究质体型ACCase基因的表达和杂草对ACCase抑制剂的抗药性机制研究提供方法学参考。

  • 相关文献

[1]基因表达分析稻瘟病菌中8个假定RhoGAP蛋白与几丁质合酶的关系. 叶文雨,豆献英,陈美莲,牛晓庆,杨雪. 2016

[2]CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii. Fei, Xiaowen,Yu, Junmei,Li, Yajun,Deng, Xiaodong. 2017

[3]Identification, characterization, and expression of a novel P450 gene encoding CYP6AE25 from the Asian corn borer, Ostrinia furnacalis. Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei,Yang, Feng-shan,Zhang, Yi-tong,Xiao, Luo. 2011

[4]TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins. Sun, Linlin,Han, Xiaori,Zhang, Shuhui,Sun, Linlin,Yu, Guohong,Xin, Shichao,Qiang, Xiaojing,Jiang, Linlin,Zhang, Shuhui,Cheng, Xianguo,Xin, Shichao.

[5]The effects of different disease-resistant cultivars of banana on rhizosphere microbial communities and enzyme activities. Sun, Jianbo,Peng, Ming,Wang, Yuguang,Li, Wenbin,Xia, Qiyu.

[6]Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium oxysporum f. sp niveum in soil. Peng, Jun,Zeng, Fanyun,Long, Haibo,Pei, Yuelin,Guo, Jianrong,Zhan, Yuanfeng. 2013

[7]Determination of ACCase Sensitivity and Gene Expression in Quizalofop-Ethyl-Resistant and -Susceptible Barnyardgrass (Echinochloa crus-galli) Biotypes. Wang, Jinxin,Wang, Jinxin. 2013

[8]Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.). Guan, L. -L.,Wang, L.,Wu, W.,Hu, B.,Li, D.,Chen, J. -W.,Hou, K.. 2014

[9]Expression of Expansin Gene (CDK-Exp3) and Its Modulation by Exogenous Gibberellic Acid During Ripening and Softening of Persimmon Fruit. Zhang, Zhengke,Fu, Runshan,Rao, Jingping,Chang, Xiaoxiao,Zhang, Yu,Jiang, Nina,Zhang, Zhengke,Hu, Meijiao,Huber, Donald J.. 2012

[10]Identification and characterization of differential gene expression in the mesocarp and kernel of oil palm nuts using suppression subtractive hybridization. Yuan, Yijun,Zhang, Lin,Wan, Li,Zheng, Yusheng,Li, Dongdong,Xu, Li,Xu, Li,Zhou, Peng,Zhou, Peng. 2011

[11]Isolation and Characterization of a Temperature-Regulated Microsomal Oleate Desaturase Gene (CtFAD2-1) from Safflower (Carthamus tinctorius L.). Guan, Ling-Liang,Xu, Ying-Wen,Wang, Yuan-Biao,Chen, Li,Shao, Jin-feng,Wu, Wei,Guan, Ling-Liang.

[12]Detection and quantification of airborne inoculum of Blumeria graminis f. sp. tritici using quantitative PCR. Cao, Xueren,Yao, Dongming,Zhou, Yilin,Fan, Jieru,Duan, Xiayu,Yao, Dongming,Ding, Kejian,West, Jonathan S.,Xu, Xiangming,Luo, Yong.

[13]用pGAP启动子在P.pastoris中组成型表达cbhⅡ基因. 易国辉,屠发志,张爱联,张添元,屈直,罗进贤. 2010

[14]高密度发酵P.pastoris诱导表达egⅡ基因. 尹慧祥,易国辉,屠发志,张添元,罗进贤,张爱联. 2011

[15]番木瓜ζ-胡萝卜素脱氢酶基因时空表达的荧光定量PCR分析. 高新征,言普,沈文涛,周鹏. 2009

[16]小麦逆境胁迫相关基因TaCBL7的克隆、生物信息学及表达特性分析. 龙翔宇,蒲至恩,郑科,刘泽厚. 2013

[17]维生素C对中华蜜蜂死亡率及其铜锌超氧化物歧化酶基因表达的影响. 刘俊峰,吴小波,王子龙,曾志将. 2013

[18]黄皮CHS基因的克隆及其表达. 张宇,许真,赵志常,高爱平,陈业渊,黄建峰,党志国,罗睿雄. 2014

[19]巴西橡胶树HbMlo1-1基因克隆及其表达. 何海霞,张宇,王萌,李晓娜,杨叶. 2016

[20]9个玉米DOF基因对逆境胁迫的响应模式分析. 贾利强,赵秋芳,陈曙. 2020

作者其他论文 更多>>