Integrated Cytological, Physiological, and Transcriptome Analyses Provide Insight into the Albino Phenotype of Chinese Plum (Prunus salicina)
文献类型： 外文期刊
作者： Xie, Weiwei 1 ; Xu, Dantong 1 ; Chen, Fangce 1 ; Wang, Zhengpeng 1 ; Luo, Jiandong 1 ; He, Yehua 1 ; Zheng, Qianming 3 ; Liu, Chaoyang 1 ;
作者机构： 1.South China Agr Univ, Coll Hort, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr & Rural Affairs, Guangzhou 510642, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming 525000, Peoples R China
3.Guizhou Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Crop Genet Resources & Germplasm Innovat K, Inst Pomol Sci, Guiyang 550006, Peoples R China
关键词： Prunus salicina; plum; albino; chloroplast development; transcriptomes
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 19 期
页码：
收录情况： SCI
摘要： Albino seedlings that arise during seed reproduction can have a significant impact on plant growth and breeding. In this research, we present the first report of albino occurrences in the seed reproduction process of Prunus salicina and describe the cytological, physiological, and transcriptomic changes observed in albino seedlings. The albino seedlings which were observed in several plum cultivars exhibited abnormal chloroplast ultrastructure and perturbed stomatal structure. Compared to normal seedlings, the photosynthetic pigment contents in albino seedlings decreased by more than 90%, accompanied by significant reductions in several chlorophyll fluorescence parameters. Furthermore, substantially changed photosynthetic parameters indicated that the photosynthetic capacity and stomatal function were impaired in albino seedlings. Additionally, the activities of the antioxidant enzyme were drastically altered against the background of higher proline and lower ascorbic acid in leaves of albino seedlings. A total of 4048 differentially expressed genes (DEGs) were identified through transcriptomic sequencing, and the downregulated DEGs in albino seedlings were greatly enriched in the pathways for photosynthetic antenna proteins and flavonoid biosynthesis. GLK1 and Ftsz were identified as candidate genes responsible for the impaired chloroplast development and division in albino seedlings. Additionally, the substantial decline in the expression levels of examined photosystem-related chloroplast genes was validated in albino seedlings. Our findings shed light on the intricate physiological and molecular mechanisms driving albino plum seedling manifestation, which will contribute to improving the reproductive and breeding efforts of plums.
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