Transcriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines
文献类型： 外文期刊
作者： Cai, Huicai 1 ; Zhang, Shuhe 1 ; Yu, Weijie 1 ; Jia, Xue 1 ; Yu, Lan 1 ; Xu, Bo 1 ; Wang, Yingzhe 2 ;
作者机构： 1.Jilin Agr Univ, Coll Forestry & Grassland Sci, Jilin Prov Key Lab Tree & Grass Genet & Breeding, Changchun, Peoples R China
2.Jilin Acad Agr Sci JAAS, Inst Agr Biotechnol, Changchun, Peoples R China
关键词： alfalfa; cytoplasmic male sterile lines; transcriptome sequencing; non-targeted metabolome sequencing; anthers
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Alfalfa (Medicago sativa L.), a prominent perennial forage in the legume family, is widely cultivated across Europe and America. Given its substantial economic value for livestock, breeding efforts have focused on developing high-yield and high-quality varieties since the discovery of CMS lines. However, progress is restricted by the limitations of existing CMS lines, necessitating the development of new lines and study of the molecular mechanisms underlying pollen abortion. This study investigates early-stage anther development in cytoplasmic male sterile (CMS) alfalfa lines (MSJN1A) in relation to the isotypic maintainer line (MSJN1B). Histological analyses revealed abnormal degradation of tapetal cells post-meiosis in the CMS line. Notably, during the early mononuclear stage, the central vacuoles in the microspores were absent, leading to evident pollen abortion. These findings suggest that pollen abortion in the CMS line is associated with the delayed disintegration of the tapetum and structural anomalies in microspore vacuoles. Non-targeted metabolome sequencing revealed 401 and 405 metabolites at late tetrad and early mononuclear stages of alfalfa, respectively. Among these, 39 metabolites were consistently upregulated, whereas 85 metabolites were downregulated. Differential analysis revealed 45 and 37 unique metabolites at each respective stage. These metabolites were primarily featured in pathways related to energy, phenylpropane, sucrose and starch, and fatty acid metabolism. Integrated analysis demonstrated that differentially expressed genes and differential metabolites were co-enriched in these pathways. Additionally, quantitative real-time PCR and physiological index analysis confirmed downregulation of key genes involved in anther development, illustrating that changes in upstream gene regulation could significantly impact downstream metabolite levels, ultimately influencing pollen fertility. Pollen abortion is related to abnormal phenylpropane metabolism, fatty acid metabolism and starch and sucrose pathway, which provides reference for further research on the causes of pollen abortion of alfalfa.
- 相关文献
作者其他论文 更多>>
-
Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.)
作者：Zhang, Shuhe;Zhang, Lin;Cai, Huicai;Zhang, Jiahao;Yang, Zhenning;Yan, Qiang;Wu, Fuju;Xu, Bo;Wang, Yingzhe
关键词：anther development; design breeding;
Medicago sativa -
Anther-specific expression of MsMYB35 transcription factor in alfalfa (Medicago sativa L.) and its crucial role in pollen development
作者：Cai, Huicai;Zhang, Shuhe;Yang, Zhenning;Zhang, Lin;Zhang, Jiahao;Zhang, Minmin;Xu, Bo;Wang, Yingzhe
关键词：Medicago sativa L; MYB transcription facto; pollen; anther development; male sterility
-
The complete genome sequences of a negative single-stranded RNA virus and a double-stranded RNA virus coinfecting the entomopathogenic fungus Beauveria bassiana Vuillemin
作者：Xu, Mengnan;Lu, Yizhuo;Zhang, Zhengkun;Liu, Jianfeng;Xu, Mengnan;Liu, Hongyu;Jia, Xue;Zou, Xiaowei;Lu, Yizhuo;Sui, Li;Li, Qiyun;Zhang, Zhengkun;Liu, Hongyu;Jia, Xue;Li, Qiyun;Zhang, Zhengkun;Li, Qiyun
关键词：
-
Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Ms beta-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)
作者：Zhou, Le;Xu, Xiaobo;Yan, Dong;Yu, Weijie;Miao, Yifan;Xu, Bo;Wang, Yingzhe
关键词：Medicago sativa L; cytoplasmic male sterility; BSA-seq; BSR-seq; DEGs
-
Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean
作者：Zhang, Ling;Wang, Yingzhe;Li, Tong;Qiu, Hongmei;Dong, Yingshan;Xia, Zhengjun
关键词：Soybean; CRISPR; Cas9; GmPDS genes; Mutation efficiency
-
Changes in phenotype and gene expression under lead stress revealed key genetic responses to lead tolerance in Medicago sativa L
作者：Yan, Dong;Xu, Xiaobo;Xu, Bo;Wang, Yingzhe;Meng, Yue;Mu, Shujing;Zhang, Ling
关键词：Medicago sativa L; Lead stress; RNA-seq; Physiological changes; Molecular mechanism
-
Genome-wide identification and characterization of the Phenylalanine Ammonia-lyase (PAL) gene family in Medicago truncatula
作者：Ren, Wei;Wang, Yingzhe;Xu, Ankai;Zhao, Yunge;Ren, Wei;Wang, Yingzhe;Xu, Ankai;Ren, Wei
关键词：Gene family; Medicago truncatula; PAL; Stress; Tissue specific
