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Functional characterization of a novel terpene synthase GaTPS1 involved in (E)-α-bergamotene biosynthesis in Gossypium arboreum

文献类型： 外文期刊

作者： Lv, Beibei 1 ; Teng, Dong 2 ; Huang, Xinzheng 4 ; Liu, Xiaohe 2 ; Liu, Danfeng 5 ; Khashaveh, Adel 2 ; Pan, Hongsheng 7 ; Zhang, Yongjun 2 ;

作者机构： 1.Shanxi Agr Univ, Inst Cotton Res, Yuncheng 044000, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Peoples R China

4.China Agr Univ, Coll Plant Protect, Beijing 100193, Peoples R China

5.Yunnan Univ, Inst Biodivers, Sch Ecol & Environm Sci, Yunnan Key Lab Plant Reprod Adaptat & Evolutionary, Kunming 650504, Peoples R China

6.Yunnan Univ, Inst Biodivers, Ctr Invas Biol, Sch Ecol & Environm Sci, Kunming 650504, Peoples R China

7.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi 830091, Peoples R China

关键词： Terpenoid biosynthesis; Herbivore induction; Enzyme catalytic activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2024 年 281 卷

页码：

收录情况： SCI

摘要： Terpenoids in plants are mainly synthesized by terpene synthases (TPSs), which play an important role in plant- environment interactions. Gossypium arboreum is one of the important cotton cultivars with excellent pest resistance, however, the biosynthesis of most terpenoids in this plant remains unknown. In this study, we performed a comparative transcriptome analysis of leaves from intact and Helicoverpa armigera-infested cotton plants. The results showed that the H. armigera infestation mainly induced the JA signaling pathway, ten TPS genes were differentially expressed in G. arboreum leaves. Among them, a novel terpene synthase, GaTPS1, was heterologously expressed and functionally characterized in vitro. . The enzymatic reaction indicated that recombinant GaTPS1 was primarily responsible for the production of (E)-alpha-bergamotene. E )-alpha-bergamotene. Moreover, molecular docking and site-directed mutagenesis analysis demonstrated that two amino acid residues, A412L and Y535F, distinctly influenced the catalytic activities and product specificity of GaTPS1. The mutants GaTPS1-A412L and GaTPS1Y535F resulted in a decrease in the proportion of products (E)-alpha-bergamotene E )-alpha-bergamotene and D-limonene, while an increase in the proportion of products (E)-(1-farnesene, E )-(1-farnesene, alpha-pinene and (1-myrcene. Our findings provide valuable insights into understanding the molecular basis of terpenoid diversity in G. arboreum , , with potential applications in plant metabolism regulation and the improvement of resistant cotton cultivars.

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