文献类型： 外文期刊
作者： Feng, Yan-Zhao 1 ; Yu, Yang 1 ;
作者机构： 1.Guangdong Acad Agr Sci, AgroBiol Gene Res Ctr, Guangzhou, Peoples R China
关键词： Circular RNA; Knock down; Overexpression; Pull-down; Quantitative PCR
期刊名称：PLANT CIRCULAR RNAS: Methods and Protocols
ISSN： 1064-3745
年卷期： 2021 年 2362 卷
页码：
收录情况： SCI
摘要： Circular RNAs (circRNAs) represent a novel group of noncoding RNA whose functions are little known in plants. Genetic manipulation is necessary for studying the biological function of specific circRNA. Here, we describe strategies to study the function of plant circRNAs including artificial microRNA-mediated circRNA knockdown, gain-of-function study, full-length circRNA identification, and circRNA-protein interaction. These methods can be applied to functional characterization of circRNAs in plants and would be promising to facilitate the research on plant circRNAs.
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