文献类型： 外文期刊

作者： Wu, Xue ¹ ; Zhang, Xiaoyan ² ; Wang, Yaya ³ ; Wu, Cuinan ¹ ; Sun, Yudong ⁴ ; Zhang, Yi ³ ; Ji, Yongran ⁵ ; Bao, Encai ¹ ; Xia, Liru ¹ ; Bian, Zhonghua ⁶ ; Cao, Kai ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Agr Minist Key Lab Agr Engn Middle & Lower Reaches, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Jiangsu, Peoples R China

3.Shanxi Agr Univ, Coll Hort, Taigu 030801, Shanxi, Peoples R China

4.Huaiyin Inst Agr Sci Xuhuai Dist Jiangsu Prov, Huaian 223001, Jiangsu, Peoples R China

5.Wageningen Univ & Res, Dept Plant Sci, Hort & Prod Physiol, NL-6700AA Wageningen, Netherlands

6.Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610299, Sichuan, Peoples R China

关键词： Watermelon; Double-root-cutting grafting; Rootstock; Adventitious root regeneration; Auxin

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）

ISSN： 2095-9885

年卷期： 2024 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting. To date, little information is available on how to enhance root regeneration of rootstock in grafted watermelon (Citrullus lanatus) seedlings. In this study, the effects of different light treatments on root regeneration were determined. This revealed that addition of far-red light (Fr) could significantly expedite root formation in the rootstock. Moreover, the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr, especially for auxin-response proteins (including CmIAA11, CmIAA17, and CmAUX28), Small auxinup RNA genes (including CmSAUR20 and CmSAUR50) and the auxin efflux transporter (CmPIN3). In addition, the expression of Phytochrome Interacting Factor (PIFs), such as CmPIF1, CmPIF3 and CmPIF7, was remarkably increased by Fr. These genes may act together to activate auxinrelated pathways under Fr treatment. Based on the results of HPLC-MS/MS analysis, the concentrations of different auxin-types in adventitious root were significantly influenced by Fr. Furthermore, the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment, which was determined by the acid magenta dyeing experiment. Therefore, all the results suggested that Fr could induce AR formation in rootstocks, which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes. The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings, which will provide a theoretical basis for the speeding development of industrialized seedlings