您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide association mapping combined with gene-based haplotype analysis identify a novel gene for shoot length in rice (Oryza sativa L.)

文献类型： 外文期刊

作者： Yang, Tifeng 1 ; Dong, Jingfang 1 ; Zhao, Junliang 1 ; Zhang, Longting 1 ; Zhou, Lian 1 ; Yang, Wu 1 ; Ma, Yamei 1 ; Wang, Jian 1 ; Fu, Hua 1 ; Chen, Jiansong 1 ; Li, Wenhui 1 ; Hu, Haifei 1 ; Jiang, Xianya 3 ; Liu, Ziqiang 2 ; Liu, Bin 1 ; Zhang, Shaohong 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Genet & Breeding High Qual Rice Southern, Guangdong Key Lab New Technol Rice Breeding, Rice Res Inst,Guangdong Rice Engn Lab,Minist Agr, Guangzhou 510640, Peoples R China

2.South China Agr Univ, Coll Agr, Guangzhou 510642, Peoples R China

3.Yangjiang Inst Agr Sci, Yangjiang 529500, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 12 期

页码：

收录情况： SCI

摘要： Key messageGenome-wide association mapping revealed a novel QTL for shoot length across multiple environments. Its causal gene, LOC_Os01g68500, was identified firstly through gene-based haplotype analysis, gene expression and knockout transgenic verification.AbstractStrong seedling vigor is an important breeding target for rice varieties used in direct seeding. Shoot length (SL) is one of the important traits associated with seedling vigor characterized by rapid growth of seedling, which enhance seedling emergence. Therefore, mining genes for SL and conducting molecular breeding help to develop varieties for direct seeding. However, few QTLs for SL have been fine mapped or cloned so far. In this study, a genome-wide association study of SL was performed in a diverse rice collection consisting of 391 accessions in two years, using phenotypes generated by different cultivation methods according to the production practice, and a total of twenty-four QTLs for SL were identified. Among them, the novel QTL qSL-1f on chromosome 1 could be stably detected across all three cultivation methods in the whole population and indica subpopulation. Through gene-based haplotype analysis of the annotated genes within the putative region of qSL-1f, and validated by gene expression and knockout transgenic experiments, LOC_Os01g68500 (i.e., Os01g0913100 in RAP-DB) was identified as the causal gene for SL, which has a single-base variation (C-to-A transversion) in its CDS region, resulting in the significant difference in SL of rice. LOC_Os01g68500 encodes a DUF538 (Domain of unknown function) containing protein, and the function of DUF538 protein gene on rice seedling growth is firstly reported in this study. These results provide a new clue for exploring the molecular mechanism regulating SL, and promising gene source for the molecular breeding in rice.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台