Isolation and Functional Characterization of a Constitutive Promoter in Upland Cotton (Gossypium hirsutum L.)
文献类型： 外文期刊
作者： Yang, Yang 1 ; Li, Xiaorong 1 ; Li, Chenyu 1 ; Zhang, Hui 1 ; Tuerxun, Zumuremu 1 ; Hui, Fengjiao 3 ; Li, Juan 1 ; Liu, Zhigang 1 ; Chen, Guo 1 ; Cai, Darun 1 ; Chen, Xunji 1 ; Li, Bo 1 ;
作者机构： 1.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol, State Key Lab Genet Improvement & Germplasm Innova, Urumqi 830091, Peoples R China
2.Xinjiang Agr Univ, Coll Agron, Urumqi 830052, Peoples R China
3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
关键词： Gossypium hirsutum L.; GUS; constitutive promoter
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 3 期
页码：
收录情况： SCI
摘要： Multiple cis-acting elements are present in promoter sequences that play critical regulatory roles in gene transcription and expression. In this study, we isolated the cotton FDH (Fiddlehead) gene promoter (pGhFDH) using a real-time reverse transcription-PCR (qRT-PCR) expression analysis and performed a cis-acting elements prediction analysis. The plant expression vector pGhFDH::GUS was constructed using the Gateway approach and was used for the genetic transformation of Arabidopsis and upland cotton plants to obtain transgenic lines. Histochemical staining and a beta-glucuronidase (GUS) activity assay showed that the GUS protein was detected in the roots, stems, leaves, inflorescences, and pods of transgenic Arabidopsis thaliana lines. Notably, high GUS activity was observed in different tissues. In the transgenic lines, high GUS activity was detected in different tissues such as leaves, stalks, buds, petals, androecium, endosperm, and fibers, where the pGhFDH-driven GUS expression levels were 3-10-fold higher compared to those under the CaMV 35S promoter at 10-30 days post-anthesis (DPA) during fiber development. The results indicate that pGhFDH can be used as an endogenous constitutive promoter to drive the expression of target genes in various cotton tissues to facilitate functional genomic studies and accelerate cotton molecular breeding.
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