文献类型： 外文期刊

作者： Son, Jun Seok ¹ ; Chae, Song Ah ² ; Zhao, Liang ² ; Wang, Hongyang ³ ; de Avila, Jeanene M. ² ; Zhu, Mei-Jun ⁴ ; Jiang, Zhihua ⁵ ; Du, Min ² ;

作者机构： 1.Univ Maryland, Dept Obstet Gynecol & Reprod Sci, Lab Perinatal Kinesioepigenet, Sch Med, Baltimore, MD 21201 USA

2.Washington State Univ, Dept Anim Sci, Nutrigen & Growth Biol Lab, Pullman, WA 99164 USA

3.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai, Peoples R China

4.Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA

5.Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA

关键词： Maternal obesity; Sarcolipin; UCP < sub > 3 <; sub >; PGC-1α Calcium activity

期刊名称：EBIOMEDICINE （ 影响因子：11.205； 五年影响因子：10.481 ）

ISSN： 2352-3964

年卷期： 2022 年 76 卷

页码：

收录情况： SCI

摘要： Background Sarcolipin and uncoupling protein 3 (UCP3) mediate muscle-based non-shivering thermogenesis (NST) to improve metabolic homeostasis. The impacts of maternal obesity (MO) and maternal exercise (ME) on NST in offspring muscle remain unexamined. Methods Female mice were fed with a control diet or high fat diet to induce obesity. Then, obese mice were further separated into two groups: obesity only (OB) and OB plus daily exercise (OB/Ex). Fetal muscle was collected at embryonic day 18.5 and offspring mice at 3-month-old. Apelin administration during pregnancy and apelin receptor (APJ) knockout mouse were further used for investigating the mediatory role of APJ on muscle-based thermogene-sis. To explore the direct effects of exercise on AMP-activated protein kinase (AMPK) downstream targets, AMPK knockout mouse was used. Findings MO inhibited while ME activated AMPK and peroxisome proliferator-activated receptor g coactivator-1a (PGC-1a) in fetal muscle. AMPK activation increased sarcolipin expression, which inhibited the uptake of calcium ions into sarcoplasmic reticulum, thereby activating CaMKK2. Consistently, the expression of UCP3 and sarcolipin was suppressed due to MO but activated in ME fetal muscle. Importantly, changes of UCP3 and sarcolipin main-tained in offspring muscle, showing the transgenerational effects. Furthermore, apelin administration during preg-nancy mimicked the effects of ME on AMPK and CaMKK2 activation, and UCP3 and sarcolipin expression, underscoring the mediatory roles of apelin-AMPK signaling in improving fetal muscle development. Interpretation ME, via activation of apelin signaling-AMPK axis, enhances NST gene expression in fetal and off -spring muscle impaired due to MO, which intergenerationally protects offspring from diet-induced obesity and met-abolic disorders. Funding This work was supported by National Institutes of Health Grant R01-HD067449. Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) eBioMedicine 103842 Published uary https://doi.org/10.1016/j. ebiom.2022.103842 Superscript/Subscript Available