LACCASE35 enhances lignification and resistance against Pseudomonas syringae pv. actinidiae infection in kiwifruit
文献类型： 外文期刊
作者： Li, Yawei 1 ; Zhang, Dongle 1 ; Wang, Xiaojie 1 ; Bai, Fuxi 3 ; Li, Rui 4 ; Zhou, Rongrong 2 ; Wu, Shunyuan 1 ; Fang, Zemin 2 ; Liu, Wei 4 ; Huang, Lili 4 ; Liu, Pu 1 ;
作者机构： 1.Anhui Agr Univ, Sch Hort, Anhui Key Lab Hort Crop Qual Biol, Hefei 230036, Peoples R China
2.Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China
3.Hubei Acad Agr Sci, Res Inst Fruit & Tea, Wuhan 430064, Peoples R China
4.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）
ISSN： 0032-0889
年卷期： 2025 年 197 卷 2 期
页码：
收录情况： SCI
摘要： Kiwifruit bacterial canker, a highly destructive disease caused by Pseudomonas syringae pv. actinidiae (Psa), seriously affects kiwifruit (Actinidia spp.) production. Lignin deposition in infected cells serves as a defense mechanism, effectively suppressing pathogen growth. However, the underlying process remains unclear. In this study, we determined that Psa infection leads to a significant increase in S-lignin accumulation in kiwifruit. The S/G ratio in lignin was higher in a Psa-resistant cultivar than in a Psa-sensitive cultivar. Furthermore, kiwifruit laccase 35 (AcLac35), encoding an enzyme in the lignin biosynthesis pathway with characteristic laccase activity, showed tissue-specific expression in plants and was upregulated following infection by Psa. Overexpressing AcLac35 in kiwifruit leaves resulted in greater lignin content than in wild-type leaves, leading to the formation of thicker cell walls, and also activated plant-pathogen interactions and MAPK pathways, thereby enhancing resistance against Psa infection. Yeast 1-hybrid assays, dual-LUC reporter assays, electrophoretic mobility shift assays, and transient injection experiments indicated that SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 9 (AcSPL9) can bind to the AcLac35 promoter, thereby positively regulating its expression. Moreover, overexpression of AcSPL9 increased lignin accumulation in kiwifruit leaves, enhancing resistance to Psa, while virus-induced gene silencing of AcSPL9 expression reduced this resistance. Our findings reveal the function of AsSPL9-AcLac35 in kiwifruit, providing insight into enhancing resistance against Psa in kiwifruit. The transcription factor AcSPL9 promotes expression of a laccase-encoding gene AcLac35, enhancing kiwifruit disease resistance and providing potential breeding targets for enhanced disease resistance.
- 相关文献
作者其他论文 更多>>
-
Identification of SepF in Streptococcus suis involving cell division
作者：Gao, Ting;Zhu, Jiajia;Liu, Wei;Yang, Keli;Zhang, Tengfei;Yuan, Fangyan;Liu, Zewen;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Li, Tingting;Zheng, Linlin;Zhou, Rui;Zheng, Linlin;Chen, Mo
关键词：
Streptococcus suis ; Cell division; Septum; Protein-protein interaction; Drug target -
Prediction of Three Sediment Phosphorus Indexes on Water Column Phosphorus Across Seasons in the Xiashan Reservoir, Northern China
作者：Liu, Wei;Hu, Fang;Fu, Songjie;Yu, Yongchao;Jiang, Shan;Liang, Lanwei;Chen, Xuemei;Jiao, Yang;Gu, Sen;Li, Qingman;Hu, Fang;Fu, Songjie;Yu, Yongchao;Jiang, Shan;Liang, Lanwei;Liu, Zhenjun;Chen, Xuemei;Jiao, Yang;Gu, Sen;Li, Qingman
关键词：sediment; phosphorus; internal loading; freshwater reservoir; risk assessment; eutrophication
-
Antibacterial activity of a plant natural poly-phenol against zoonotic Streptococcus suis
作者：Gao, Ting;Wang, Yanjun;Zhu, Jiajia;Chen, Mo;Yang, Keli;Yuan, Fangyan;Liu, Zewen;Liu, Wei;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Wang, Yanjun;Li, Lu;Chen, Mo;Tian, Haibin
关键词：Streptococcus suis; Plant resource; Poly-phenol; Biofilm; Antibiotic alternative; Antibiotic adjuvant
-
Preparation and Evaluation of Novel Epitope-Based ETEC K88-K99 Bivalent Vaccine
作者：Wang, Shuangshuang;Yue, Xinru;Bei, Weicheng;Wang, Shuangshuang;Yue, Xinru;Bei, Weicheng;Wang, Shuangshuang;Yue, Xinru;Bei, Weicheng;Wang, Shuangshuang;Yang, Yuxin;Yue, Xinru;Liu, Zewen;Yuan, Fangyan;Yang, Keli;Zhu, Jiajia;Liu, Wei;Tian, Yongxiang;Wu, Qiong;Gao, Ting;Li, Chang;Song, Haofei;Zhou, Danna
关键词：Enterotoxigenic Escherichia coli; FaeG; FanC; epitope; vaccine
-
Rapeseed Green Manure Coupled with Biochar and Vermicompost Enhances Soil Aggregates and Fungal Communities in Gleyed Paddy Fields
作者：Zhu, Zhenhao;Gao, Shihong;Zhang, Yuhao;Si, Guohan;Xu, Xiangyu;Peng, Chenglin;Zhao, Shujun;Liu, Wei;Zhu, Zhenhao;Gao, Shihong;Zhu, Qiang;Geng, Mingjian;Zhang, Yuhao
关键词：gleyed paddy fields; rapeseed green manure; biochar; vermicompost; soil aggregate stability; fungal community
-
Prevalence, Molecular Characterization, and Antimicrobial Resistance Profile of Enterotoxigenic Escherichia coli Isolates from Pig Farms in China
作者：Zhu, Jiajia;Liu, Zewen;Wang, Siyi;Gao, Ting;Liu, Wei;Yang, Keli;Yuan, Fangyan;Wu, Qiong;Li, Chang;Guo, Rui;Tian, Yongxiang;Zhou, Danna;Wang, Siyi
关键词：Enterotoxigenic
Escherichia coli ; MLST; serotypes; virulence genes; minimum inhibitory concentration; antimicrobial resistance genes -
Bacillus spp. Potentiate the Virulence and Intracellular Invasion of A. paragallinarum in Chickens
作者：Zhu, Jiajia;Gao, Ting;Yang, Keli;Liu, Wei;Zhou, Danna;Liu, Ying;Chen, Yunsheng;Zhu, Kui
关键词：
Bacillus spp.;A. paragallinarum ; coinfection; bacterial interaction; NAD; antibiotic resistance
