Comparative Transcriptome Profiling Reveals Two WRKY Transcription Factors Positively Regulating Polysaccharide Biosynthesis in Polygonatum cyrtonema
文献类型： 外文期刊
作者： Jiang, Wu 1 ; Chen, Jiadong 1 ; Duan, Xiaojing 1 ; Li, Yaping 2 ; Tao, Zhengming 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China
2.Zhejiang A&F Univ, Zhejiang Prov Key Lab Resources Protect & Innovat, Hangzhou 311300, Peoples R China
关键词： polysaccharide biosynthesis; regulatory mechanism; germplasms; transcriptome; Polygonatum cyrtonema
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 16 期
页码：
收录情况： SCI
摘要： Polygonatum cyrtonema (P. cyrtonema) is a valuable rhizome-propagating traditional Chinese medical herb. Polysaccharides (PCPs) are the major bioactive constituents in P. cyrtonema. However, the molecular basis of PCP biosynthesis in P. cyrtonema remains unknown. In this study, we measured the PCP contents of 11 wild P. cyrtonema germplasms. The results showed that PCP content was the highest in Lishui Qingyuan (LSQY, 11.84%) and the lowest in Hangzhou Lin'an (HZLA, 7.18%). We next analyzed the transcriptome profiles of LSQY and HZLA. Through a qRT-PCR analysis of five differential expression genes from the PCP biosynthesis pathway, phosphomannomutase, UDP-glucose 4-epimerase (galE), and GDP-mannose 4,6-dehydratase were determined as the key enzymes. A protein of a key gene, galE1, was localized in the chloroplast. The PCP content in the transiently overexpressed galE1 tobacco leaves was higher than in the wild type. Moreover, luciferase and Y1H assays indicated that PcWRKY31 and PcWRKY34 could activate galE1 by binding to its promoter. Our research uncovers the novel regulatory mechanism of PCP biosynthesis in P. cyrtonema and is critical to molecular-assisted breeding.
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