Comparative Transcriptome Profiling Reveals Two WRKY Transcription Factors Positively Regulating Polysaccharide Biosynthesis in Polygonatum cyrtonema
文献类型： 外文期刊
作者： Jiang, Wu 1 ; Chen, Jiadong 1 ; Duan, Xiaojing 1 ; Li, Yaping 2 ; Tao, Zhengming 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China
2.Zhejiang A&F Univ, Zhejiang Prov Key Lab Resources Protect & Innovat, Hangzhou 311300, Peoples R China
关键词： polysaccharide biosynthesis; regulatory mechanism; germplasms; transcriptome; Polygonatum cyrtonema
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 16 期
页码：
收录情况： SCI
摘要： Polygonatum cyrtonema (P. cyrtonema) is a valuable rhizome-propagating traditional Chinese medical herb. Polysaccharides (PCPs) are the major bioactive constituents in P. cyrtonema. However, the molecular basis of PCP biosynthesis in P. cyrtonema remains unknown. In this study, we measured the PCP contents of 11 wild P. cyrtonema germplasms. The results showed that PCP content was the highest in Lishui Qingyuan (LSQY, 11.84%) and the lowest in Hangzhou Lin'an (HZLA, 7.18%). We next analyzed the transcriptome profiles of LSQY and HZLA. Through a qRT-PCR analysis of five differential expression genes from the PCP biosynthesis pathway, phosphomannomutase, UDP-glucose 4-epimerase (galE), and GDP-mannose 4,6-dehydratase were determined as the key enzymes. A protein of a key gene, galE1, was localized in the chloroplast. The PCP content in the transiently overexpressed galE1 tobacco leaves was higher than in the wild type. Moreover, luciferase and Y1H assays indicated that PcWRKY31 and PcWRKY34 could activate galE1 by binding to its promoter. Our research uncovers the novel regulatory mechanism of PCP biosynthesis in P. cyrtonema and is critical to molecular-assisted breeding.
- 相关文献
作者其他论文 更多>>
-
A Mycorrhiza-Induced UDP-Glucosyl Transferase Negatively Regulates the Arbuscular Mycorrhizal Symbiosis
作者：Chen, Jiadong;Zhao, Qingchun;Xie, Kun;Wang, Mengna;Li, Lechuan;Zeng, Dechao;Wang, Qiuli;Wang, Shuangshuang;Chen, Aiqun;Xu, Guohua;Chen, Jiadong;Chen, Aiqun;Xu, Guohua
关键词：arbuscular mycorrhiza; flavonoid; glycosyltransferase; soluble sugars
-
Untargeted Metabolomics Reveals the Metabolic Characteristics and Biomarkers of Antioxidant Properties of Gardeniae Fructus from Different Geographical Origins in China
作者：Jiang, Wu;Duan, Xiaojing;Chen, Jiadong;Liu, Yingying;Tao, Zhengming;Jiang, Wu;Duan, Xiaojing;Chen, Jiadong;Liu, Yingying;Tao, Zhengming;Jiang, Lingling;Jiang, Lingling;Yin, Xiaoli;Zhang, Shuhui;Zheng, Hong
关键词：antioxidant; biomarker; metabolomics; flavonoids; geographical origin
-
Putative Upstream Regulators DoNF-YB3 and DoIDD12 Correlate with DoGSTF11 Expression and Anthocyanin Accumulation in Dendrobium officinale
作者：Liu, Yingying;Chen, Jiadong;Duan, Xiaojing;Zhang, Man;Tao, Zhengming;Jiang, Wu;Liu, Yingying;Chen, Jiadong;Duan, Xiaojing;Zhang, Man;Tao, Zhengming;Jiang, Wu
关键词：anthocyanins;
Dendrobium officinale ;DoGSTF11 ; regulatory mechanism -
The cellulose synthase-like G3 (CslG3) gene mediates polysaccharide synthesis and drought stress response in Dendrobium catenatum
作者：Hou, Hongyan;Wu, Junxia;Zhang, Yu;Lu, Chenfei;Shao, Qingsong;Lv, Aimin;Pan, Lanying;Jiang, Wu;Shen, Yanghui
关键词：Dendrobium catenatum; Cellulose synthase-like (Csl); Polysaccharide biosynthesis; Abiotic stresses; Gene family analysis
-
Genome-Wide Identification of Glutathione S-Transferase Family from Dendrobium officinale and the Functional Characterization of DoGST5 in Cadmium Tolerance
作者：Jiang, Wu;Wang, Tao;Zhang, Man;Guo, Qiaosheng;Jiang, Wu;Duan, Xiaojing;Chen, Jiadong;Liu, Yingying;Tao, Zhengming
关键词：Dendrobium officinale; glutathione S-transferase; cadmium tolerance; reactive oxygen species
-
Transcription factor DcbZIPs regulate secondary metabolism in Dendrobium catenatum during cold stress
作者：Zhou, Xiaohui;Lu, Chenfei;Zhou, Aicun;Lv, Aimin;Shao, Qingsong;Pan, Lanying;Zhou, Xiaohui;Lu, Chenfei;Zhou, Fenfen;Zhu, Yanqin;Lv, Aimin;Shao, Qingsong;Jiang, Wu;Zhou, Aicun;Shen, Yanghui
关键词：bZIP; cold stress; Dendrobium catenatum; secondary metabolism; transcription regulation
-
A novel polysaccharide from Rubus chingii Hu unripe fruits: Extraction optimization, structural characterization and amelioration of colonic inflammation and oxidative stress
作者：Luo, Hanqi;Ying, Na;Zhao, Qihui;Chen, Junli;Xu, Hangying;Gao, Hongchang;Zheng, Hong;Jiang, Wu;Wu, Yaozong;Wu, Yinliang
关键词：Raspberry; Polysaccharide; Colon; Inflammation; Gut microbiota
