Putative Upstream Regulators DoNF-YB3 and DoIDD12 Correlate with DoGSTF11 Expression and Anthocyanin Accumulation in Dendrobium officinale
文献类型： 外文期刊
作者： Liu, Yingying 1 ; Chen, Jiadong 1 ; Duan, Xiaojing 1 ; Zhang, Man 1 ; Tao, Zhengming 1 ; Jiang, Wu 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou 325005, Peoples R China
2.Zhejiang Acad Agr Sci, Innovat Ctr Chinese Med Crops, Hangzhou 310021, Peoples R China
关键词：
anthocyanins;
期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 11 卷 6 期
页码：
收录情况： SCI
摘要： Dendrobium officinale is a traditional and valuable medicinal herb, with extensive research conducted on its polysaccharides, alkaloids, and other components, yet studies on anthocyanins remain limited. In this study, we analyzed the expression levels of GST family genes in green and purplish D. officinale and found that DoGSTF11 is highly expressed in the purplish variety. DoGSTF11 is localized to the nucleus and cell membrane but lacks transcriptional activation activity. Overexpression of DoGSTF11 in tomato enhances anthocyanin accumulation, suggesting a role in anthocyanin sequestration or transport. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays further revealed that DoGSTF11 interacts with DoGST31, while DoIDD12 and DoNF-YB3 are potential transcriptional regulators based on promoter-binding assays and expression correlation. In conclusion, our study demonstrates that DoGST11 positively regulates anthocyanin accumulation in D. officinale. These findings provide valuable insights into the metabolic engineering of flavonoids in D. officinale.
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