文献类型： 外文期刊

作者： Wang, Yihan ¹ ; Li, Zhaoyu ¹ ; Ji, Chun ² ; Wang, Yanhua ³ ; Chu, Zunhua ⁴ ; Zhang, Tianliang ⁴ ; Chen, Chen ¹ ;

作者机构： 1.Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Jinan 250012, Peoples R China

2.TongZhou Dist Comprehens Inspection & Testing Ctr, Nantong 226300, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

4.Shandong Ctr Dis Control & Prevent, Shandong Ctr Food Safety Risk Assessment, Jinan 250014, Peoples R China

关键词： Opsariichthys bidens; Beta-cypermethrin; Pyraclostrobin; Combined effect; Toxic mechanism; Molecular docking

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：7.6； 五年影响因子：8.3 ）

ISSN： 0269-7491

年卷期： 2024 年 358 卷

页码：

收录情况： SCI

摘要： The extensive utilization of pesticides results in their frequent detection in aquatic environments, often as complex mixtures, posing risks to aquatic organisms. The hook snout carp ( Opsariichthys bidens ) serves as a valuable bioindicator for evaluating the impacts of environmental pollutants in aquatic ecosystems. However, few studies examined the toxic effects of pesticides on O.bidens , let alone the characterization of the combined effects resulting from their mixtures. This study aims to elucidate the toxic effects of beta-cypermethrin and pyraclostrobin on O.bidens , individually and in combination, focusing on biochemical, transcriptional, and molecular responses. By organizing and analyzing the toxicogenomic databases, both pesticides were identified as a contributor to processes such as apoptosis, oxidative stress, and inflammatory responses. The acute toxicity test revealed comparable acute toxicity of beta-cypermethrin and pyraclostrobin on O.bidens , with LC 50 being 0.019 and 0.027 mg/L, respectively, whereas the LC 50 decreased to 0.0057 and 0.0079 mg/L under the combined exposure, indicating potential synergistic effects. The activities of enzymes involved in oxidative stress and detoxification were significantly altered after exposure, with superoxide dismutase (SOD) and catalase (CAT) increasing, while malondialdehyde (MDA) levels decreased. The activity of CYP450s was significantly changed. Likewise, the expression levels of genes ( mn-sod , p53 , esr , il-8 ) associated with oxidative stress, apoptosis, endocrine and immune systems were significantly increased. Combined exposure to the pesticides significantly exacerbated the aforementioned biological processes in O.bidens . Furthermore, both pesticides can modify protein activity by binding to the surface of SOD molecules and altering protein conformation, contributing to the elevated enzyme activity. Through the investigation of the synergistic toxic effects of pesticides and molecular mechanisms in O.bidens , our findings highlight the importance of assessing the combined effects of pesticide mixtures in aquatic environments.