文献类型： 外文期刊
作者： Hu, Rui 1 ; Liang, Kaiming 1 ; Hu, Xiangyu 1 ; Li, Meijuan 1 ; Ye, Qunhuan 1 ; Yin, Yuanhong 1 ; Tang, Cai 1 ; Wang, Xinyu 1 ; Fu, Youqiang 1 ; Pan, Junfeng 1 ; Zhang, Mingyong 2 ; Zhong, Xuhua 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Guangdong Acad Agr Sci, Key Lab Genet & Breeding High Qual Rice Southern C, Guangdong Rice Engn Lab,Guangdong Key Lab Rice Sci, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China
关键词： asparagine; amino acid metabolism; endosperm development; rice
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 13 期
页码：
收录情况： SCI
摘要： Seed development plays a critical role in determining both crop yield and grain quality in rice. As a key nutrient storage organ, the rice endosperm development not only contributes to grain filling but also plays an essential role during the early stages of seed germination. Amino acid metabolism is active during the process of seed development and seed germination. Asparagine is a primary amino acid responsible for long-distance organic nitrogen transport in plants. Asparagine synthetase catalyzes the synthesis of asparagine from aspartate and glutamine. In this study, CRISPR/Cas9-mediated knockout mutants of the OsASN2 gene of rice were generated. Homozygous mutants exhibited complete failure of seed germination, and heterozygotes could not produce homozygous offspring. Endosperm development of homozygous mutant seeds showed severe defects. Additionally, interacting protein screening combined with pull-down and co-immunoprecipitation (Co-IP) assays confirmed that OsASN2 physically interacted with pyruvate phosphate dikinase OsPPDKB, the mutants of which showed impaired endosperm development. These findings collectively indicate that OsASN2 plays a critical role in seed development and germination in rice.
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