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Ratiometric fluorescence and photothermal dual-mode immunosensor based on MnO2 nanosheets for the detection of isocarbophos

文献类型： 外文期刊

作者： Liu, Jie 1 ; Deng, Hao 2 ; Jia, Bao-Zhu 3 ; Lin, Ze-Shan 4 ; Wang, Yu 4 ; Wang, Hong 1 ; Xu, Zhen-Lin 1 ; Luo, Lin 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

2.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold chain Hainan P, Haikou 571100, Peoples R China

3.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Peoples R China

4.Guangzhou Inst Food Inspect, Guangzhou 510080, Peoples R China

关键词： Dual-mode immunosensor; Ratiometric fluorescence; Photothermal detection; Manganese dioxide nanosheets

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）

ISSN： 1385-8947

年卷期： 2024 年 502 卷

页码：

收录情况： SCI

摘要： Isocarbophos (ICP) is a typical highly toxic organophosphorus pesticide, and its rapid and accurate detection is essential for food safety. In this study, we presented a ratiometric fluorescence and photothermal dual-mode immunoassay for the quantification of ICP in food samples, leveraging the oxidase-like activity and photothermal effect of manganese dioxide nanosheets (MnO2NSs). The immunoassay utilized alkaline phosphatase (ALP) as a labeling enzyme, which efficiently catalyzes the dephosphorylation of ascorbic acid 2-phosphate (AA2P) to produce ascorbic acid (AA). The generated AA subsequently initiated the decomposition of MnO2NSs, resulting in a reduction of both the photothermal signal from MnO2NSs and the fluorescence signal from oxidized Amplex Red (oxAR), while moderately restoring the fluorescence emission from blue-emissive carbon dots (b-CDs). The limits of detection (LOD) were as low as 2.62 pg mL- 1 in photothermal mode and 0.21 pg mL- 1 in ratiometric fluorescence mode. Recovery rates from spiked food samples were 94.8-119.8 % in photothermal mode and 97.9-105.5 % in ratiometric fluorescence mode. This study creates promising new avenues for pesticide detection and establishes an effective platform for dual-mode immunosensors.

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