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Effect of supplemetation of Zebularine and Scriptaid on efficiency of in vitro developmental competence of ovine somatic cell nuclear transferred embryos

文献类型： 外文期刊

作者： Fang, Xiaohuan 1 ; Xia, Wei 2 ; Cao, Hui 1 ; Guo, Yanhua 3 ; Wang, Han 4 ; Zhang, Xiaosheng 5 ; Wan, Pengcheng 3 ; Liu, Chuang 1 ; Wei, Qiaoli 1 ; Sun, Shuchun 1 ; Tian, Shujun 1 ; Li, Junjie 1 ; Wang, Zhigang 1 ;

作者机构： 1.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China

2.Southwest Minzu Univ, Coll Life Sci & Technol, Chengdu, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China

4.Hebei Agr Univ, Coll Food Sci & Technol, Baoding, Peoples R China

5.Anim Husb & Vet Res Inst Tianjin, Tianjin, Peoples R China

6.Res Ctr Cattle & Sheep Embryo Engn Tech Hebei Pro, Baoding, Peoples R China

关键词： Sheep; somatic cell nuclear; transfer; brilliant cresyl blue; Zebularine; Scriptaid

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）

ISSN： 1049-5398

年卷期： 2020 年 31 卷 2 期

页码：

收录情况： SCI

摘要： Somatic cell nuclear transfer (SCNT) technology has been applied in the construction of disease model, production of transgenic animals, therapeutic cloning, and other fields. However, the cloning efficiency remains limited. In our study, to improve SCNT efficiency, brilliant cresyl blue (BCB) staining were chosen to select recipient oocytes. In addition, DNA methyltransferase inhibitor Zebularine (5nmol/L) and histone deacetylase inhibitor Scriptaid (0.2 mu mol/L) were jointly used to treat sheep donor cumulus cells and reconstructed embryo. Moreover, the expression levels of embryonic development-related genes (OCT4, SOX2, H19, IGF2 and Dnmt1) of reconstructed embryo were also detected. Using BCBthornoocytes as recipient cell, donor cumulus cells and reconstructed embryos were treated with 5nmol/L Zebularine and 0.2 mu mol/L Scriptaid, the blastocyst rate in ZebthornSCR-SCNT group (28.25%) was significantly higher than SCNT (21.16%) (p< 0.05). Furthermore, results showed that expression levels of OCT4, SOX2, H19, IGF2 and Dnmt1 genes in ZebthornSCR-SCNT embryos were more similar to IVF embryos. Our study proved that 5nmol/L Zebularine and 0.2 mu mol/ L Scriptaid treating with sheep donor cumulus cells and reconstructed embryos improved SCNT blastocyst rate and relieve the abnormal expression of embryonic developmental related genes.

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