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Efficient production of extracellular alkaline protease in Bacillus amyloliquefaciens by host strain construction

文献类型： 外文期刊

作者： Chen, Weijie 1 ; Li, Lu 2 ; Ye, Changwen 3 ; Zhao, Ziyue 1 ; Huang, Kuo 3 ; Zou, Dian 1 ; Wei, Xuetuan 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan 430070, Peoples R China

2.Guangdong Acad Agr Sci, Sericultural & Argi Food Res Inst, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Minist Agr & Rural, Guangzhou 510610, Peoples R China

3.China Natl Tobacco Corp, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China

关键词： B; amyloliquefaciens; Gene knock-out; Host modification; Alkaline protease

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.056； 五年影响因子：6.295 ）

ISSN： 0023-6438

年卷期： 2022 年 163 卷

页码：

收录情况： SCI

摘要： Bacillus amyloliquefaciens is an important industrial microorganism, which can be applied as a protein expression host. However, the extracellular protein expression ability of B. amyloliquefaciens is still low. Hence, it is necessary to develop a B. amyloliquefaciens host with a high extracellular protein expression capacity. In this study, B. amyloliquefaciens HZ-12 was the original strain, then seven important extracellular protease genes (epr, nprE, aprE-a, mpr, pbpF, vpr, ykct1), one important intracellular protease gene (aprX) and two redundant proteins genes (htrB, hag) were deleted in HZ-12, resulting in B. amyloliquefaciens BAX-10. In order to evaluate the extracellular protein expression ability of BAX-10, the alkaline protease gene aprE from Bacillus subtilis D7 was expressed in BAX-10. After fermentation, the alkaline protease activity of BAX-10/aprE reached 666.82 U/mL, which was 57% higher than that of the control strain HZ/aprE. Moreover, the deletion of the ten genes had no negative effect on the growth of B. amyloliquefaciens. In summary, B. amyloliquefaciens BAX-10 could be used as a potential platform host for expression of target extracellular proteins.

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