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Female-Biased Odorant Receptor MmedOR48 in the Parasitoid Microplitis mediator Broadly Tunes to Plant Volatiles

文献类型： 外文期刊

作者： Li, Ruijun 1 ; Song, Xuan 1 ; Shan, Shuang 2 ; Hussain Dhiloo, Khalid 2 ; Wang, Shanning 5 ; Yin, Zixuan 2 ; Lu, Ziyun 6 ; Khashaveh, Adel 2 ; Zhang, Yongjun 2 ;

作者机构： 1.Hebei Agr Univ, Coll Plant Protect, Baoding 071000, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

3.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing 100193, Peoples R China

4.Sindh Agr Univ, Fac Crop Protect, Dept Entomol, Tandojam 70060, Pakistan

5.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Fruit, Beijing 100097, Peoples R China

6.Hebei Acad Agr & Forestry Sci, IPM Ctr Hebei Prov, Key Lab Integrated Pest Management Crops Northern, Minist Agr & Rural Affairs, Baoding 071000, Hebei, Peoples R China

关键词： parasitoid wasp; odorantreceptor; TEVC recordings; ligand-binding profile; molecular docking; electrophysiological response

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.7； 五年影响因子：6.0 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 31 期

页码：

收录情况： SCI

摘要： Odorant receptors (ORs) play a crucial role in insect chemoreception. Here, a female-biased odorant receptor MmedOR48 in parasitoid Microplitis mediator was fully functionally characterized. The qPCR analysis suggested that the expression level of MmedOR48 increased significantly after adult emergence and was expressed much more in the antennae. Moreover, an in situ hybridization assay showed MmedOR48 was extensively located in the olfactory sensory neurons. In two-electrode voltage clamp recordings, recombinant MmedOR48 was broadly tuned to 23 kinds of volatiles, among which five plant aldehyde volatiles excited the strongest current recording values. Subsequent molecular docking analysis coupled with site-directed mutagenesis demonstrated that key amino acid residues Thr142, Gln80, Gln282, and Thr312 together formed the binding site in the active pocket for the typical aldehyde ligands. Furthermore, ligands of MmedOR48 could stimulate electrophysiological activities in female adults of the M. mediator. The main aldehyde ligand, nonanal, aroused significant behavioral preference of M. mediator in females than in males. These findings suggest that MmedOR48 may be involved in the recognition of plant volatiles in M. mediator, which provides valuable insight into understanding the olfactory mechanisms of parasitoids.

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