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Molecular characterization and expression of sensory neuron membrane proteins in the parasitoid Microplitis mediator (Hymenoptera: Braconidae)

文献类型： 外文期刊

作者： Shan, Shuang 1 ; Wang, Shan-Ning 2 ; Song, Xuan 2 ; Khashaveh, Adel 2 ; Lu, Zi-Yun 5 ; Dhiloo, Khalid Hussain 6 ; Li, 1 ;

作者机构： 1.China Agr Univ, Coll Plant Protect, Beijing, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China

4.Agr Univ Hebei, Coll Plant Protect, Baoding, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Minist Agr, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov,Plant Protect Inst, Baoding, Peoples R China

6.Sindh Agr Univ, Fac Crop Protect, Dept Entomol, Tandojam, Pakistan

关键词： antennae; chemosensation; expression profile; in situ hybridization; Microplitis mediator; sensory neuron membrane proteins

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2020 年 27 卷 3 期

页码：

收录情况： SCI

摘要： Sensory neuron membrane proteins (SNMPs), homologs of the human fatty acid transport protein CD36 family, are observed to play a significant role in chemoreception, especially in detecting sex pheromone in Drosophila and some lepidopteran species. In the current study, two full-length SNMP transcripts, MmedSNMP1 and MmedSNMP2, were identified in the parasitoid Microplitis mediator (Hymenoptera: Braconidae). Quantitative real-time polymerase chain reaction analysis showed that the expression of MmedSNMP1 was significantly higher in antennae than in other tissues of both sexes. In addition, the MmedSNMP1 transcript was increased dramatically in newly emerged adults and there were no significant differences between adults with or without mating and parasitic experiences. However, compared with MmedSNMP1, the expression of MmedSNMP2 was widely found in various tissues, significantly increased at half-pigmented pupae stage and remained at a relatively constant level during the following developmental stages. It was found that MmedSNMP1 contained eight exons and seven introns, which was highly conserved compared with other insect species. In situ hybridization assay demonstrated that MmedSNMP1 transcript was distributed widely in antennal flagella. Among selected chemosensory genes (odorant binding protein, odorant receptor, and ionotropic receptor genes), MmedSNMP1 only partially overlapped with MmedORco in olfactory sensory neurons of antennae. Subsequent immunolocalization results further indicated that MmedSNMP1 was mainly expressed in sensilla placodea of antennae and possibly involved in perceiving plant volatiles and sex pheromones. These findings lay a foundation for further investigating the roles of SNMPs in the chemosensation of parasitoids.

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