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Cryo-EM structure reveals cylindrical nucleocapsids from two polydnaviruses

文献类型: 外文期刊

作者: Cui, Ji-Hui 1 ; Chen, Ya-Bin 1 ; Li, Ming 1 ; Cai, Qiu-Chen 1 ; Zhang, Li-Dan 1 ; Lu, Zi-Yun 4 ; Li, Jian-Cheng 4 ; Zhu, 1 ;

作者机构: 1.Yunnan Univ, Sch Life Sci, Kunming, Yunnan, Peoples R China

2.Yunnan Univ, Key Lab Biochem & Mol Biol High Educ Yunnan Prov, Kunming, Yunnan, Peoples R China

3.Yunnan Univ, Key Lab Anim Genet Divers & Evolut High Educ Yunn, Kunming, Yunnan, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Hebei, Peoples R China

5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

ISSN: 0304-8608

年卷期: 2018 年 163 卷 12 期

页码:

收录情况: SCI

摘要: Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernable cap structures in two distal regions with relatively high electron density. Adjacent to the end-cap structures are two electron-lucent rings. Some nucleocapsids were uniformly electron-dense and had a distinctive helix-tail-like structure. Cryo-EM revealed inconsistent nucleocapsid diameters of 34-69.9nm in MbBV and 46-69.9nm in MmBV, and the largest observed cylindrical area length was expanded to 126nm.

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