Effect of Estradiol on Estrogen Nuclear Receptors Genes Expression on Embryonic Development Stages in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
文献类型： 外文期刊
作者： Chen, Guobin 1 ; Zhou, Tong 1 ; Chen, Meng 1 ; Zou, Guiwei 1 ; Liang, Hongwei 1 ;
作者机构： 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： Chinese soft-shelled turtle (Pelodiscus sinensis); sexual reversal; estrogen receptors; estrogen
期刊名称：FISHES （ 影响因子：3.17； 五年影响因子：3.098 ）
ISSN：
年卷期： 2022 年 7 卷 5 期
页码：
收录情况： SCI
摘要： Among Chinese soft-shelled turtles, Pelodiscus sinensis, males have a richer nutritional value and higher market price than females. All-male offspring were obtained by 17 beta-estradiol (E2). However, the molecular mechanisms of E2 inducing sexual reversal remain unclear. In this study, we cloned estrogen nuclear receptors (ERs) from P. sinensis and investigated their expression profiles. We examined the responses of ER alpha and ER beta after treatment with different concentrations of 1.0, 5.0, and 10 mg/mL E2. ERs showed abundant expressions in the adult gonad, ER alpha for ovary, and ER beta for testis. E2 can up-regulate the expression of ER alpha, which showed a remarkable increase while the promotion of ER beta was unobvious. They reached a high level at stage 17 after the treatment of E2, genes of the female-related genes Rspo1, Wnt4, beta-catenin, Foxl2, Cyp19a1, and Sox3 exhibited a significant raise at stage 17 with the increase in the concentration of E2 while the male-related genes Sox9, Dmrt1, and Amh were significantly inhibited. Our study cloned the full length of ERs and analyzed their structures and expressions, laying a foundation for the further study of the effect of estrogen on sex determination.
- 相关文献
作者其他论文 更多>>
-
Targeting and editing the second exon of bmp6 gene results in a silver carp with reduced intramuscular bones
作者：Niu, Shuang;Li, Xiaohui;Feng, Cui;Zhang, Zewen;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei;Liang, Hongwei
关键词：Intermuscular bones; bmp6; Hypophthalmichthys molitrix; Transcriptome analysis
-
Antioxidant Capacity and Disease Resistance Enhanced by Dietary D-Glucuronolactone Supplementation in Chinese Soft-Shelled Turtles (Pelodiscus sinensis)
作者：Zhou, Tong;Xue, Mingyang;Zhou, Yong;Liang, Hongwei;Liu, Wei;Wu, Wenyi;Xue, Mingyang
关键词：aquaculture; D-glucuronolactone; antioxidant; immune response; health;
Pelodiscus sinensis ; feeding trial -
Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts
作者：Li, Hangyu;Liang, Hongwei;Gao, Xiaowen;Zeng, Xiangtong;Zheng, Shuo;Wang, Linlin;Yuan, Faming;Xu, Shaohua;Yin, Zhan;Hu, Guangfu;Liang, Hongwei;Yin, Zhan
关键词：satiety neuropeptide; follicle-stimulating hormone; GnRH3; pituitary; sexual maturity
-
Chromosome-scale genome assembly and annotation of Xenocypris argentea
作者：Wu, Yidi;Sha, Hang;Liang, Hongwei;Liang, Hongwei
关键词：
-
A 5′ Promoter Region SNP in CTSC Leads to Increased Hypoxia Tolerance in Changfeng Silver Carp (Hypophthalmichthys molitrix)
作者：Feng, Nannan;Zhang, Jiquan;Feng, Nannan;Li, Xiaohui;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei;Liang, Hongwei
关键词：
Hypophthalmichthys molitrix ; hypoxia; SNP;CTSC ; HIF-1 alpha -
METTL14 facilitates the process of sexual reversal via m6A RNA methylation in Pelodiscus sinensis
作者：Zhou, Tong;Chen, Guobin;Cao, Jizeng;Ji, Huizi;Zou, Guiwei;Liang, Hongwei;Cao, Jizeng;Ji, Huizi
关键词：METTL14; Pelodiscus sinensis; RNA methylation; Sexual reversal
-
Effects of Different Carbohydrate Levels in Diets on Growth Performance and Muscle Nutritive Value of Ying Carp and Scattered-Scaled Mirror Carp (Cyprinus carpio)
作者：Xiao, Pengfei;Xiao, Pengfei;Wu, Yunya;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei
关键词：
