Effect of Estradiol on Estrogen Nuclear Receptors Genes Expression on Embryonic Development Stages in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
文献类型： 外文期刊
作者： Chen, Guobin 1 ; Zhou, Tong 1 ; Chen, Meng 1 ; Zou, Guiwei 1 ; Liang, Hongwei 1 ;
作者机构： 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： Chinese soft-shelled turtle (Pelodiscus sinensis); sexual reversal; estrogen receptors; estrogen
期刊名称：FISHES （ 影响因子：3.17； 五年影响因子：3.098 ）
ISSN：
年卷期： 2022 年 7 卷 5 期
页码：
收录情况： SCI
摘要： Among Chinese soft-shelled turtles, Pelodiscus sinensis, males have a richer nutritional value and higher market price than females. All-male offspring were obtained by 17 beta-estradiol (E2). However, the molecular mechanisms of E2 inducing sexual reversal remain unclear. In this study, we cloned estrogen nuclear receptors (ERs) from P. sinensis and investigated their expression profiles. We examined the responses of ER alpha and ER beta after treatment with different concentrations of 1.0, 5.0, and 10 mg/mL E2. ERs showed abundant expressions in the adult gonad, ER alpha for ovary, and ER beta for testis. E2 can up-regulate the expression of ER alpha, which showed a remarkable increase while the promotion of ER beta was unobvious. They reached a high level at stage 17 after the treatment of E2, genes of the female-related genes Rspo1, Wnt4, beta-catenin, Foxl2, Cyp19a1, and Sox3 exhibited a significant raise at stage 17 with the increase in the concentration of E2 while the male-related genes Sox9, Dmrt1, and Amh were significantly inhibited. Our study cloned the full length of ERs and analyzed their structures and expressions, laying a foundation for the further study of the effect of estrogen on sex determination.
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