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Cytochrome P450 CYP6EM1 Underpins Dinotefuran Resistance in the Whitefly Bemisia tabaci

文献类型： 外文期刊

作者： Huang, Mingjiao 1 ; Fu, Buli 2 ; Yin, Cheng 2 ; Gong, Peipan 2 ; Liu, Shaonan 1 ; Yang, Jing 2 ; Wei, Xuegao 2 ; Liang, Jinjin 2 ; Xue, Hu 2 ; He, Chao 2 ; Du, Tianhua 2 ; Wang, Chao 2 ; Ji, Yao 1 ; Hu, Jinyu 2 ; Zhang, Rong 2 ; Du, He 2 ; Zhang, Youjun 1 ; Yang, Xin 2 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Minist Agr & Rural Affairs Key Lab Integrated Pes, Haikou 571101, Hainan, Peoples R China

4.Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Managem, Jingzhou 434025, Hubei, Peoples R China

关键词： Bemisia tabaci; cytochrome P450; dinotefuran; insecticide resistance; resistance management

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 10 期

页码：

收录情况： SCI

摘要： Being a destructive pest worldwide, the whitefly Bemisia tabaci has evolved resistance to neonicotinoid insecticides. The third-generation neonicotinoid dinotefuran has commonly been applied to the control of the whitefly, but its underlying mechanism is currently unknown. On the base of our transcriptome data, here we aim to investigate whether the cytochrome P450 CYP6EM1 underlies dinotefuran resistance in the whitefly. Compared to the susceptible strain, the CYP6EM1 gene was found to be highly expressed in both laboratory and field dinotefuran-resistant populations. Upon exposure to dinotefuran, the mRNA levels of CYP6EM1 were increased. These results demonstrate the involvement of this gene in dinotefuran resistance. Loss and gain of functional studies in vivo were conducted through RNAi and transgenic Drosophila melanogaster assays, confirming the role of CYP6EM1 in conferring such resistance. In a metabolism assay in vitro, the CYP6EM1 protein could metabolize 28.11% of dinotefuran with a possible dinotefuran-dm-NNO metabolite via UPLC-QTOF/MS. Docking of dinotefuran to the CYP6EM1 protein showed a good binding affinity, with an energy of less than -6.0 kcal/mol. Overall, these results provide compelling evidence that CYP6EM1 plays a crucial role in the metabolic resistance of B. tabaci to dinotefuran. Our work provides new insights into the mechanism underlying neonicotinoid resistance and applied knowledge that can contribute to sustainable control of a global pest such as whitefly.

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