您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Glutathione S-transferase directly metabolizes imidacloprid in the whitefly, Bemisia tabaci

文献类型： 外文期刊

作者： Zhang, Rong 1 ; Yang, Jing 2 ; Hu, Jinyu 1 ; Yang, Fengbo 1 ; Liang, Jinjin 2 ; Xue, Hu 2 ; Wei, Xuegao 1 ; Fu, Buli 2 ; Huang, Mingjiao 2 ; Du, He 2 ; Wang, Chao 1 ; Su, Qi 1 ; Yang, Xin 2 ; Zhang, Youjun 2 ;

作者机构： 1.Yangtze Univ, Coll Agr, Minist Agr & Rural Affairs, Hubei Engn Technol Ctr Forewarning & Management Ag, Jingzhou 434025, Hubei, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

3.Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China

4.Chinese Acad Trop Agr Sci, Minist Agr & Rural Affairs, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Peoples R China

关键词： Bemisia tabaci; Imidacloprid; Glutathione S-transferase; Metabolism

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0048-3575

年卷期： 2024 年 201 卷

页码：

收录情况： SCI

摘要： The whitefly Bemisia tabaci poses a significant threat to various crops and ornamental plants and causes severe damage to the agricultural industry. Over the past few decades, B. tabaci has developed resistance to several pesticides, including imidacloprid. Therefore, elucidating the mechanism that leads to insecticide detoxification is very important for controlling B. tabaci and managing whitefly resistance to neonicotinoid insecticides. Among insect detoxification enzymes, glutathione S-transferase (GST) is an important phase II detoxification enzyme that helps detoxify exogenous toxic substances. In this study, we cloned the BtGSTz1 gene and observed that its expression level was greater in imidacloprid-resistant populations than sensitive populations of B. tabaci. By silencing BtGSTz1 via RNA interference, we found a significant increase in the mortality of imidacloprid-resistant B. tabaci. Additionally, prokaryotic expression and in vitro metabolism studies revealed that the recombinant BtGSTz1 protein could metabolize 36.36% of the total imidacloprid, providing direct evidence that BtGSTz1 plays a crucial role in the detoxification of imidacloprid. Overall, our study elucidated the role of GSTs in physiological activities related to insecticide resistance, which helps clarify the resistance mechanisms conferred by GSTs and provides useful insights for sustainable integrated pest management.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台