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DNA methylation mediated by RdDM pathway and demethylation affects furanone accumulation through regulation of QUINONE OXIDOREDUCTASE in strawberry

文献类型： 外文期刊

作者： Li, Yunduan 1 ; Shi, Yanna 1 ; Li, Yichen 1 ; Lu, Jiao 1 ; Sun, Yunfan 1 ; Zhang, Yuanyuan 1 ; Chen, Wenbo 1 ; Yang, Xiaofang 4 ; Grierson, Donald 1 ; Lang, Zhaobo 6 ; Jiang, Guihua 4 ; Chen, Kunsong 1 ;

作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Zijingang Campus, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Peoples R China

3.Zhejiang Univ, State Agr Minist Lab Hort Plant Growth Dev & Qual, Zijingang Campus, Hangzhou 310058, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

5.Univ Nottingham, Sch Biosci, Div Plant & Crop Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen 518055, Peoples R China

7.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Recently, increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening. However, the role of DNA methylation in regulating specific traits, such as flavor, remains unclear. Here, we report a role of DNA methylation in affecting furanone biosynthesis in strawberry. Strawberry quinone oxidoreductase (FaQR) is a key enzyme in furanone biosynthesis. There are four FaQR homologs in strawberry cultivar 'Yuexin', and one of them, FaQR3, contributes similar to 50% of FaQR transcripts, indicating a major role of FaQR3 in furanone biosynthesis. Through characterization of levels of DNA methylation and FaQR3 transcript and furanone contents during fruit ripening and after the application of DNA methylation inhibitor, we found that the DNA methylation level of the FaQR3 promoter was negatively correlated with FaQR3 expression and furanone accumulation, suggesting that DNA methylation may be involved in furanone biosynthesis through adjusting FaQR3 expression, and responded to different temperatures consistently. In addition, transient expression of a gene in the RNA-directed DNA methylation (RdDM) pathway, FaAG04, and enrichment analysis of the 24-nucleotide siRNAs suggested that DNA methylation in the FaQR3 promoter is mediated by the RdDM pathway. Transient RNA interference (RNAi) of FaDML indicated that the demethylation pathway may be involved in regulating furanone accumulation. These findings provide new insights into the role of DNA methylation and demethylation in affecting flavor quality in strawberry during fruit ripening.

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